雷公藤甲素对人结肠癌HCT116细胞增殖、自噬和凋亡的影响  

Effect of triptolide on human colorectal cancer HCT116 cell proliferation,autophagy and apoptosis

作　　者：赵林 吴鹏 章平贵 谢大泽 高典[3] 周南进 

机构地区：[1]江西省医学科学院实验中心,江西南昌330006 [2]江西省医学科学院分子医学研究所,江西南昌330006 [3]南昌大学基础医学院,江西南昌330006

出　　处：《中国药理学通报》2016年第10期1399-1404,共6页Chinese Pharmacological Bulletin

基　　金：江西省自然科学基金资助项目(No 20142BAB205079)

摘　　要：目的探究雷公藤甲素(TP)对人结肠癌HCT116细胞增殖的的影响和可能的抑制机制。方法取TP不同浓度、不同时间作用HCT116细胞,MTT检测细胞的存活率,流式细胞术检测TP对细胞凋亡的影响,免疫印迹检测蛋白LC3-Ⅱ表达,MDC检测细胞自噬。结果 TP能够抑制HCT116细胞的增殖,不同浓度的TP作用HCT116细胞48 h后,LC3-Ⅱ的水平随着TP浓度的增加而升高,呈浓度依赖性;用40nmol·L^(-1)TP作用HCT116细胞,LC3-Ⅱ蛋白水平随作用时间的延长而增加,呈时间依赖性,同时荧光显微镜观察到HCT116细胞中酸性自噬囊泡的数量增多。TP+3-MA作用HCT116细胞48 h,与对照组比较TP组细胞凋亡率下降;与TP组比较TP+3-MA组细胞凋亡率明显下降。TP+RAPA作用HCT116细胞24 h,与对照组比较TP组细胞凋亡率增加;与TP组比较TP+RAPA组细胞凋亡率明显增加。结论TP能够抑制人结肠癌HCT116细胞的增殖,诱导HCT116细胞凋亡,提示自噬和凋亡间可能有协同关系。Aim To investigate the effect of triptolide （ TP） on human colorectal cancer HCT116 cell prolif-eration and explore the potential mechanism of TP in treating colon cancer. Methods MTT assay was used for estimating the survival rates of HCT116 cells ex-posed to different concentrations and different duration time of TP. Western blot （ WB ） was used for testing the expression of LC3-Ⅱ. MDC staining was employed to detect cell autophagy. Flow cytometry （ FCM） was applied to test the effects of TP on cell apoptosis. Re-sults TP could inhibit cell proliferation in HCT116 cells. The expression of LC3-Ⅱwas enhanced with the increase of the concentration of TP after exposed to dif-ferent concentrations of TP for 48 h and the duration time of TP after exposed to 40 nmol·L^-1 TP,and the number of acid autophagic vacuoles in HCT116 cells had increased, which was observed by fluorescence mi-croscope. The HCT116 cells apoptotic rates in TP group had decreased compared to control group and de-creased significantly compared to TP ＋3-MA group. The HCT116 cells apoptotic rates in TP group had in-creased compared to control group and increased signif-icantly compared to TP＋RAPA group. Conclusion TP could inhibit the proliferation of human colon canc-er HCT116 cells and induce apoptosis in HCT116 cells, which indicates that synergy may exist between autophagy and apoptosis.

关 键 词：雷公藤甲素 结肠癌 HCT116细胞 增殖 自噬 凋亡 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]