黄芪甲苷对缺氧缺糖/复氧复糖PC12细胞凋亡的影响  

Effects of astragaloside Ⅳ on apoptosis of PC12 cells induced by hypoxia/hypoglycemia and reoxygenation

作　　者：靳晓飞[1] 张颖[2] 周晓红[2] 武密山[2] 赵艳萌 高维娟[2] 

机构地区：[1]承德医学院病理生理学教研室,河北承德067000 [2]河北中医学院河北省心脑血管病中医药防治重点实验室,河北石家庄050200

出　　处：《中国药理学通报》2016年第10期1411-1415,共5页Chinese Pharmacological Bulletin

基　　金：河北省应用基础研究计划重点项目(No 16967756D);河北省普通高校高层次人才科学研究计划项目(No GCC2014031);河北省重点学科建设项目

摘　　要：目的观察黄芪甲苷对缺氧缺糖/复氧复糖PC12细胞凋亡的影响。方法取对数生长期的PC12细胞,随机分为4组:正常对照组、缺氧缺糖/复氧复糖组、黄芪甲苷组和黄芪甲苷溶剂对照组。其中缺氧缺糖/复氧复糖组、黄芪甲苷组和黄芪甲苷溶剂对照组进行缺氧缺糖3h后再复氧复糖12 h,并于复氧复糖的同时加入黄芪甲苷。用倒置显微镜观察细胞形态,MTT法检测细胞活性,TUNEL染色和Annexin V-FITC/PI流式细胞术检测细胞凋亡情况。结果正常对照组PC12细胞贴壁生长,呈多角形,突起明显,突起之间交织成网状,细胞膜光滑且完整,胞体折光性较强。与正常对照组相比,缺氧缺糖/复氧复糖组细胞变圆或肿胀,突起回缩或消失,细胞膜破损,胞体折光性差,细胞活性明显下降(P<0.05),细胞凋亡率和凋亡指数明显升高(P<0.05)。与缺氧缺糖/复氧复糖组相比,黄芪甲苷组细胞生长状态见好转,突起较为明显,胞体折光性较好,细胞活性升高(P<0.05),细胞凋亡率和凋亡指数下降(P<0.05)。黄芪甲苷溶剂对照组与缺氧缺糖/复氧复糖组相比无明显差异(P>0.05)。结论黄芪甲苷可减轻缺氧缺糖/复氧复糖引起的PC12细胞损伤,提高细胞活性,抑制细胞凋亡。Aim To investigate the effects of astragalo-side IV on apoptosis of PC12 cells inducedby hypoxia/hypoglycemia and reoxygenation. Metheds PC12 cells were randomly divided into 4 groups： normal control group,hypoxia/hypoglycemia and reoxygenation group, astragaloside Ⅳ group and vehicle group. Hypoxia/hy-poglycemia and reoxygenation group, astragaloside Ⅳgroup and vehiclegroup were exposed to reoxygenation （12 h） after 3 h of oxygen and glucose deprivation, and astragaloside Ⅳ was added into cells at the same time. Inverted microscope was used to observe the morphological changes ofPC12 cells and MTT method to detect the activities of PC12 cells, and Annexin V-FITC/PI assay and TUNEL staining were used to meas-ure the apoptosis of PC12 cells. Results Compared with normal control group, cells became round or swol-len and its cellula processes were retracted or disap-peared in hypoxia/hypoglycemia and reoxygenation group;a large number of apoptotic cells could also be observed,whose nucleus were shrinkaged, fragmented or deep-stained. The activities of hypoxia/hypoglycemia and reoxygenation group were decreased markedly than those in normalcontrol group（P〈0. 05）,while the ap-optotic rates of hypoxia/hypoglycemia and reoxygen-ation group were increased obviously than those in nor-malcontrol group（ P〈0. 05 ） . Compared with hypoxia/hypoglycemia and reoxygenation group, a good cell growth state could be observed and cellula processes could also be observed significantly in astragaloside Ⅳgroup. The activities of astragaloside Ⅳ group were in-creased than those in hypoxia/hypoglycemia and reoxy-genation group（P〈0. 05）,while the apoptotic rates of astragalosideⅣgroup were decreased than those in hy-poxia/hypoglycemia and reoxygenation group （ P 〈0. 05 ） . There was no obvious difference between vehi-clegroup and hypoxia/hypoglycemia and reoxygenation group（ P 〉0. 05 ） . Conclusion Astragaloside IV can reduce the damage of PC12 cells induced by hypoxia/hypoglycemia and reoxyg

关 键 词：缺氧缺糖/复氧复糖 黄芪甲苷 PC12细胞 细胞凋亡 TUNEL染色 AnnexinV-FITC/PI流式细胞术 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]