广西北部地区汉坦病毒基因型研究  

Genotypes of hantanvirus in north areas of Guangxi

作　　者：闭福银[1] 谭毅[1] 莫建军[1] 谢镇国[1] 陈敏玫[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《应用预防医学》2016年第5期383-385,397,共4页Applied Preventive Medicine

基　　金：广西疾病预防控制中心课题(201103)

摘　　要：目的：查明广西北部地区汉坦病毒的基因型及其基因亚型。方法用RT-PCR方法检测鼠肺中的汉坦病毒并对M和S片段进行扩增和测序。下载已知毒株序列并进行比较，以明确其基因型及其亚型。结果2011年采集宿主动物肺组织标本356份，RT-PCR 检测阳性12份。S 片段与汉城型汉坦病毒（SEOV）的同源性较高，在86.6%-88.9%之间，与汉滩型汉坦病毒（HTNV）的同源性较低，在73.5%-74.0%之间。系统进化分析显示属于汉城型汉坦病毒的S6亚型。结论广西北部地区汉坦病毒基因型为汉城型汉坦病毒S6亚型。Objective To investigate the genetypes and sub-genetypes of hantavirus in the north areas of Guangxi. Methods Hantavirus in the lung tissues of wild rats was identified by RT-PCR,and the complete S and pairtial M segments of positive samples were amplified with RT-PCR and sequenced. Phylogenetic analysis was used for genotyping. Results Three hundred and fifty-six specimens of rat lung tissues were collected in 2011, and 12 were positive with RT-PCR testing . The homology of complete S segments with other hantavirus SEOV and HTNV clades were 86.6%-88.9% and 73.5%-74.0%,respectively.Phylogenetic analysis of pairtial M segments showed that hantaviruses in north Guangxi was SEOV ,and the sub-genotype was S6. Conclusion The genotype of hantaviruses in north Guangxi was SEOV,and the sub-genotype was S6.

关 键 词：汉坦病毒 基因型 基因亚型 

分 类 号：R373.32[医药卫生—病原生物学]