维甲酸受体在小鼠胚胎造血发育中的表达  

Expression of retinoic acid receptor in the mouse embryonic hematopoiesis

作　　者：李亚梅[1] 张文君[2] 汪俊帮 江凡[1] 张炎[1] 曹雨娜 梁爱斌[2] 张虹[1] LI Ya-mei ZHANG Wen-jun WANG Jun-bang JIANG Fan ZHANG Yan CAO Yu-na LIANG Ai-bin ZHANG Hong(Department of Clinical Pharmacology, Tongji Hospital, Tongji University, Shanghai 200065, China Department of Hematology, Tongji Hospital, Tongji University, Shanghai 200065, China)

机构地区：[1]同济大学附属同济医院临床药理室,上海200065 [2]同济大学附属同济医院血液科,上海200065

出　　处：《第二军医大学学报》2016年第10期1245-1249,共5页Academic Journal of Second Military Medical University

摘　　要：目的 检测小鼠胚胎造血发育中维甲酸（retinoic acid,RA）受体（RA receptors,RARs）不同亚型RAR-α、RAR-β、RAR-γ及其合成酶视黄醛脱氢酶1（retinal dehydrogenase 1,Raldh1）和Raldh2的转录水平,探讨RARs不同亚型及其合成酶在胚胎造血发育中的作用。方法 获取孕鼠胎龄9.5d（embryonic day 9.5,E9.5）卵黄囊（yolk sac,YS）,E10.5、E11.5主动脉-性腺-中肾（aorta-gonad-mesonephros,AGM）区,E13.5、E14.5、E17.5胎肝（fetal liver,FL）和成年小鼠骨髓（bone marrow,BM）,运用实时荧光定量PCR检测各组织中RAR-α、RAR-β、RAR-γ、Raldh1和Raldh2的转录水平。结果 与其他不同阶段造血组织比较,在E9.5 YS、E11.5 AGM区和BM中,RAR-α、RAR-β、RAR-γ及Raldh1、Raldh2均高表达（P〈0.01）。E11.5 AGM区RAR-α、RAR-β、RAR-γ及Raldh1、Raldh2表达水平高于E10.5 AGM区（P〈0.01）。而E13.5-E17.5FL间的RAR-β、RAR-γ及Raldh1、Raldh2表达水平差异无统计学意义。结论YS、AGM区和BM中RAR-α、RAR-β、RAR-γ及其合成酶Raldh1和Raldh2表达趋势与已有研究中相应造血组织来源的红系造血祖细胞改变相一致,反映了RA与小鼠个体发育中YS、AGM区和BM的造血形成密切相关,而RA对FL的造血形成作用可能并不显著。Objective To investigate the expression of retinoic acid receptors （RARs） subtypes RAR-α, RAR-β, RAR-γ and retinal dehydrogenases 1 （Raldh1 ） , Raldh2 in the mouse embryonic hematopoiesis, so as to discuss the role of different RAR subtypes in embryonic hematopoiesis. Methods The yolk sac （YS ） at embryonic day 9. 5 （E9. 5）,aorta-gonad- mesonephros （AGM） region at E10. 5 and E1. 5,fetal liver （FL） at E13. 5, E14. 5 and E17. 5 were harvested from the pregnant mice. The bone marrow （BM） was also harvested from adult mice. The mRNA levels of RAR-α, RAR-β, RAR-γ ,Raldh1 and Raldh2 at the above four different hematopoietic sites were assessed by quantitative reverse transcription polymerase chain reaction （RT-PCR）. Results The mRNA levels of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 at E9.5 YS , E11.5 AGM regions and BM were significantly higher than those in other relevant hematopoietic tissues （P〈0.01）. Compared with the E10. 5 AGM region, E11. 5 AGM region had significantly increased mRNA levels of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 （P〈0. 01）, but the mRNA levels of these genes in FL at E13. 5-E17. 5 were not significantly different. Conclusion The expressions of RAR-α, RAR-β, RAR-γ, Raldh1 and Raldh2 in YS, AGM regions and BM are consistent with the situation of colony forming unit erythroid （CFU-E）,suggesting that retinoic acid is closely related to the formation of hematopoiesis in the YS, AGM region and BM in mouse, but has less correlation with the FL in mouse.

关 键 词：维甲酸 维甲酸受体 造血系统 胚胎发育 视黄醛脱氢酶 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学]