病毒感染复数对狂犬病毒PM株增殖的影响  

Effect of MOI on proliferation of rabies virus PM strain in human diploid 2BS cells

作　　者：陈智[1] 孙艳[1] 牟建超[1] 刘莉娜[1] 刘宇[1] 刘辉[1] 刘杰[1] 

机构地区：[1]成都生物制品研究所有限责任公司病毒性疫苗研究室,四川成都610023

出　　处：《微生物学免疫学进展》2016年第5期30-33,共4页Progress In Microbiology and Immunology

摘　　要：目的：探讨不同感染复数（Multiplicity of infection,MOI）的狂犬病毒PM株在人二倍体细胞（2BS株）中增殖的影响,确定感染复数。方法将狂犬病毒PM株按照MOI 0.01、0.02、0.05、0.10、0.15、0.20接种至2BS细胞中,用显微镜观察接种病毒后细胞的形态变化；培养3-5d后,第一次收获病毒液；更换培养液继续培养3-4d后,进行第二次收获。采用小鼠脑内滴定法测定狂犬病毒滴度,通过NIH法测定灭活病毒液的效价。结果当MOI为0.05-0.10时,细胞圆缩30%-40%,细胞能维持较好的生长形态,可收获病毒,病毒收获液的滴度较高（〉5.0 lgLD50/mL）,且灭活后病毒液的效价较高（〉4.0IU/mL）。结论确定狂犬病毒PM株在人二倍体2BS细胞增殖的适宜MOI,为人用狂犬病疫苗生产工艺的优化提供了依据。Objective To investigate the effect from MOI to the proliferation for rabies virus PM strain on human diploid 2BS cells. Methods 2BS cells were inoculated with rabies virus PM strain at MOI of 0. 01, 0. 02, 0. 05, 0. 10, 0. 15 and 0. 20, respectively, after inoculation the cell morphology was observed by a microscopy. The first harvest for cultured virus was obtained 3-5 days after inoculation, and the second harvest in 3-4 days after a continuous cultivation by a replacement medium. The titer of harvested virus was determined by the mouse brain inoculation test, and the potency of inactivated vi-rus was determined by the test in NIH mice. Results When inoculated at MOI 0. 05-0. 10, the cell shrinkage was from 30% to 40%, the cells could be maintained in a good status and havested. The harvested rabies virus was maintaned a high titer （〉5. 0 lgLD50/mL）, and the inactivated virus obtained a high potency （〉4. 0 IU/mL）. Conclusion The appropri-ate MOI was determined on the proliferation for rabies virus PM strain on human diploid 2BS cells, which provides a basis in optimization of the rabies vaccine production process.

关 键 词：狂犬病毒(PM株) 2BS细胞 感染复数 病毒滴度 效价 

分 类 号：R373.9[医药卫生—病原生物学]