肉毒毒素体外检测方法的研究进展  

Advances in botulinum toxin detection method in vitro

作　　者：叶婷[1] 张雪平[1] 

机构地区：[1]兰州生物制品研究所有限责任公司毒素制剂室甘肃省疫苗工程技术研究中心,甘肃兰州730046

出　　处：《微生物学免疫学进展》2016年第5期57-63,共7页Progress In Microbiology and Immunology

摘　　要：肉毒毒素是肉毒梭状芽胞杆菌在生长繁殖过程中产生的一种神经毒素，对人和动物具有强毒性和高致死性，在世界范围内，肉毒中毒的案例时有发生，病情严重者一般2-3d即可死亡。肉毒毒素产品所用的体内检测方法（小鼠生物法）的结果变异系数大，不利于肉毒毒素产品含量的标准化控制。因而，建立准确且灵敏度高的肉毒毒素体外检测方法，在临床治疗及制品的质量控制方面都具有重要意义，现就常用的肉毒毒素体外检测方法作一阐述。Botulinum toxin is a neurotoxin generated in the growth of the clostridium botulinum, which has an extremely tox-icity and seriously lethal effect for human and animal. Botulism cases frequently occur in the worldwide, few serious pa-tients will die in two to three days. In addition, the detection method for botulinum toxin product is carried out the mouse bioassay in vivo which has a higher coefficient of variations, so it is not conducive to the standardization of qality control for content of botulinum toxin product. Therefore, it has a great significance to set up accurate and sensitive detection methods in vitro in clinical treatment of botulism and quality control of botulinum toxin product. The paper summarizes the latest progress in detection methods for botulinum toxin in vitro.

关 键 词：肉毒毒素 小鼠生物法 体外检测 细胞学检测 质谱法 

分 类 号：R99[医药卫生—毒理学]