熏洗Ⅰ号对大鼠瘘管术后创面微血管生成及微循环的影响  

Effect of Fumigating ＆ Washing No. 1 on Rats Wound Repairment after Operation for Fistula in Angiogenesis and Microcirculation

作　　者：谢贻祥[1] 王传思[1] 郑学海[1] 姚磊 黄鸿武[1] 王永森[1] 吴永军[1] 

机构地区：[1]安徽医科大学附属六安医院肛肠外科,安徽六安237005

出　　处：《中国肛肠病杂志》2016年第8期7-10,共4页Chinese Journal of Coloproctology

基　　金：安徽省高校自然科学基金项目（KJ20132144）;安徽省卫生计生委中医药科研课题项目（2014zy53）

摘　　要：为探讨熏洗Ⅰ号对大鼠瘘管术后创面微循环及微血管生成的影响，本研究将60只雄性SD大鼠随机分为熏洗Ⅰ号组、高锰酸钾组、生理盐水组和空白对照组，每组15只，前3组建立瘘管术后动物模型，造模成功后，分别应用中药熏洗Ⅰ号纱条、1：5000高锰酸钾溶液纱条、生理盐水纱条湿敷创面，每次30rain，每天早晚各1次，空白对照组正常喂养，不予特殊处理；并分别于术后第1天、第7天和第14天从各组中取5只大鼠检测创面血流情况，然后将其处死，采集标本，应用免疫组化法检测创面肉芽组织中血管内皮生长因子（VEGF）及CD34表达情况。结果显示，术后第1天，熏洗Ⅰ号组、高锰酸钾组创面血流量明显高于空白对照组和生理盐水组，P〈0．05；熏洗Ⅰ号组与高锰酸钾组比较，差异无统计学意义，P〉0．05。术后第7天，熏洗Ⅰ号组、高锰酸钾组创面血流量明显高于空白对照组和生理盐水组，P〈0．05；熏洗Ⅰ号组明显高于高锰酸钾组，P〈0．05。术后第14天，熏洗Ⅰ号组、高锰酸钾组创面血流量明显低于空白对照组和生理盐水组，P〈0．05；熏洗Ⅰ号组明显低于高锰酸钾组，P〈0．05。术后大鼠瘘管创面组织中VEGF及CD34表达均明显增高，并基本随着术后创面恢复时间的增加而呈现递增趋势。熏洗Ⅰ号组中VEGF和CD34表达水平明显高于生理盐水组及高锰酸钾组，P〈0．05。结果表明，熏洗Ⅰ号对大鼠瘘管术后创面局部血流及创面肉芽组织中VEGF、CD34表达有较好的调节作用，可改善局部微循环，提高创面愈合质量。This study was to explore the effect of fumigating ＆ washing No.1 on rat wound repairment after operation for fistula in angiogenesis and microcirculation; 60 male Sprague-Dawley（SD） rats were divided into four groups, fumigating ＆washing No.1 group, potassium permanganate（PP） group, normal saline group and blank control group, 15 rats for each.The former three groups were established animal model of postoperation of fistula.After the success of modeling, the gauze strips of fumigating ＆ washing No. 1 of Chinese medicine, 1 ： 5000 PP solution, normal saline were applied to wet dress wound respectively,30 min per time, once respectively in the morning and evening;the blank control group were feed regularly without any particular treatment.Five rats were selected from each group and detected the situation of wound bleed- ing after the 1st day, 7th day, and 14th day of operation respectively; then put them to death and collected specimen.The expression of vascular endothelial growth factor （VEGF） and CD34 in wound granulation tissue was detected by the immunohistochemical method.The results showed that on the 1st clay and the 7th day of postoperation,the fumigating ＆ washing No.1 group and PP group were obviously higher than the blank control group and normal saline group in the volume of wound bleeding （ P 〈0.05）.Comparing fumigating ＆ washing No.1 group and PP group on the 1st day of postoperation,there was no statistic significance in difference （ P 〉0.05）.But on the 7th day of postoperation,the fumigating ＆ washing No. 1 group was obviously higher than PP group （ P 〈0.05）. On the 14th day of postoperation, fumigating ＆washing No.1 group and PP group were obviously lower than blank control group and normal saline group in the volume of wound bleeding （ P〈0.05） and fumigating ＆ washing No. 1 group was obviously lower than PP group （ P 〈0.05）.In rats wound tissue of fistula after surgery,the expression of VEGF and CD34 was all obviously increased; basicall

关 键 词：熏洗Ⅰ号 瘘管 微循环 血管内皮生长因子 CD34 大鼠 

分 类 号：R697.120.7[医药卫生—泌尿科学]