外科医用胶栓堵兔子宫的效果研究  

Study on effect of surgical adhesive to occlude rabbit uterus

作　　者：徐安然[1] 郝天羽[1] 韦晓晴[2] 李传芬[3] 牛力华[1] 张书芬[4] 邓晓惠[5] 

机构地区：[1]济南军区总医院生殖医学中心,250031 [2]山东省济南市妇幼保健院生殖医学中心 [3]济南军区总医院神经内科实验室 [4]山东中医药大学第二附属医院妇产科 [5]山东大学齐鲁医院不孕不育诊疗中心

出　　处：《中国计划生育学杂志》2016年第10期656-659,共4页Chinese Journal of Family Planning

摘　　要：目的:考察外科医用胶对兔子宫的栓堵效果及子宫组织形态学变化,为临床体外受精-胚胎移植(IVF-ET)前病变输卵管的处理提供实验依据。方法:将35只新西兰成年雌兔随机分为实验组(23只)和对照组(12只),实验组于开腹手术下行外科医用胶双侧近输卵管处子宫栓堵术;对照组仅行开腹手术(假手术),不进行医用胶栓堵。每组分别于术后1个月、6个月观察体重变化、子宫组织学变化,比较子宫肌层厚度,进行子宫输卵管造影检查评价子宫的栓堵情况。结果:35只兔成功手术且术后正常存活31只(实验组20只、对照组11只);两组术后1个月和6个月体重比较均无统计学差异;术后1个月和6个月实验组子宫堵塞率分别为95.00%(19/20)、85.00%(17/20),高于对照组,均有统计学差异(P<0.05);实验组不同时间点组内子宫堵塞率比较无统计学差异。子宫肌层厚度,实验组术后1个月(374.19±67.02μm)和6个月(957.43±95.55μm)比较有统计学差异(P=0.000);术后1个月实验组与对照组比较有统计学差异(P=0.000);术后6个月实验组与对照组比较无统计学差异(P=0.182)。结论:外科医用胶对兔子宫栓堵有效,组织形态学上无明显有害影响。此胶用于临床上IVF-ET前病变输卵管的栓堵具有可行性。Objective： To investigate the occlusion effect of surgical medical adhesive on the rabbit uterus and the histo- morphology change of theuterus after occlusion, and to provide experimental basis for the clinical treatment of abnormal fallopian tube before IVF-ET. Method： 35 adult female New Zealand rabbits were randomly divided into the exper imental group （n = 23） and the control group （n = 12）. The experimental group received laparotomy and uterus oc clusion by surgical adhesive adjacent to both fallopian tubes. The control group only received laparotomy （sham）. The change of body weight, histological of the uterine, and myometrium thickness were observed, and hysterosalpingogra phy techniques were used to evaluate the uterus occlusion at 1 and 6 months after surgical. Result： Among the 35 rab bits, 31 were successfully operated and survived, of which 20 belonged to the experimental group and 11 were from the control group. There was no significant difference in mortality between the two groups, and the body weight between the two groups after 1 and 6 months showed no significant difference. The difference of myometrium thickness was statistically significant （P=0.000） in the experimental group at the two time points. The difference between groups after 1 month was statistically significant （P=0.000）, and was no statistically significant after 6 months （P=0.182）. Conclusion： Surgical medical adhesive is effective in occluding uterus of rabbits, and shows no obvious harmful effect on the uterus in histomorphology. In theory, the adhesive is feasible for the occlusion in the abnormal fallopian tube in clinic before IVF-ET.

关 键 词：Α-氰基丙烯酸酯 外科医用胶 子宫 栓堵 兔 

分 类 号：R714.8[医药卫生—妇产科学]