基于DNA条形码和子宫骨片形态特征的8种雌性麻蝇种类鉴定  

Identification of 8 female Sarcopha flies based on mitochondrial DNA and signum morphological characteristics

作　　者：岳巧云[1,2] 邱德义 单振菊 汪小东 胡佳 张文庆[1] 

机构地区：[1]中山大学有害生物控制与资源利用国家重点实验室,广东广州510275 [2]中山出入境检验检疫局检验检疫技术中心,广东中山528403

出　　处：《中国媒介生物学及控制杂志》2016年第5期436-442,共7页Chinese Journal of Vector Biology and Control

基　　金：国家科技支撑计划课题(2012BAK11B05);国家质检总局项目(2015IK067;2015IK069);广东省科技项目(2015A050502009)~~

摘　　要：目的建立一种准确鉴定雌性麻蝇物种的可靠方法。方法 2011年10月25日至2013年10月27日,共收集35对正在交配的8种麻蝇及57只雄性个体并获取其DNA条形码序列,依据雄性麻蝇的尾器特征进行种类鉴定;从GenBank下载11条该8种麻蝇序列,比较种内所有个体、雌雄个体及不同种类间DNA条形码序列的差异度。雌性个体的种类根据与其交配的雄性个体,并结合DNA条形码确定,详细描述8种雌性麻蝇子宫骨片的主要形态特征,并依据其物证编写种类鉴定检索表。结果 8种麻蝇的同种雌雄个体间DNA条形码序列差异范围为0-1.37%,与同种个体间的差异范围相符,且〈2%的DNA条形码种类鉴定的界限,不同种类间DNA条形码差异范围为4.56%-8.81%,与种内不同个体间有明显差异。不同种类雌性麻蝇的子宫骨片结构具有种的特异性,可作为雌性麻蝇种类鉴定的依据。结论 DNA条形码可准确地鉴定雌性个体,并进一步发展雌性的某种独特的形态特性,为实现雌性个体的直接形态鉴定提供依据。Objective To establish a method to accurately identify the species of the female Sarcopha. Methods Thirty five mating pairs of eight Sarc@ha species and 57 single male individuals of these 8 species were collected from October 25 in 2011 to October 27 in 2013. Their DNA barcodes were sequenced and 11 DNA barcodes sequences of these 8 species were also downloaded from the GenBank. Males were identified according to their genitalia, DNA barcodes differences between the intra-species, inter-sexes and inter-species were compared. Females were identified according to their mating males and the DNA barcodes, female＇ s signum morphological characteristics of these eight species were illustrated and a key based on it was constructed. Results Inter-sexes DNA barcodes differences were 0-1.37% of these 8 species, and were comparable to the intra-species variations, and were lower than the 2% species limitation. The inter-species DNA barcodes differences were 4.56%-8.81%, there are distinct gaps between the intra-spccies and inter-species. The signum morphological characteristics were species-specific and could be used to identify the females. Conclusion DNA barcoding is a practical tool to identify the female Sarcopha. Species-specific morphological characters of the female could be developed based on the identification result of DNA barcoding, then provides references for the direct morphological identification of the females.

关 键 词：雌性麻蝇 DNA条形码 子宫骨片形态特征 

分 类 号：R384.2[医药卫生—医学寄生虫学]