DNA条形码技术在中国-哈萨克斯坦边境阿拉山口地区鼠类鉴定中的应用  

Application of DNA barcoding in classification of rodents at Alataw pass, China-Kazakhstan

作　　者：王安东[1,2] 尹小平[2] 田延河[2] 梁臻[2] 巴特[2] 张江国[2] 

机构地区：[1]新疆石河子大学动物科技学院,新疆石河子832000 [2]阿拉山口出入境检验检疫局,新疆博乐833418

出　　处：《中国媒介生物学及控制杂志》2016年第5期459-462,共4页Chinese Journal of Vector Biology and Control

基　　金：国家质检总局科技计划项目(2016IK264)~~

摘　　要：目的以中国-哈萨克斯坦边境(中哈边境)阿拉山口地区常见鼠种为研究对象,使用线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)和细胞色素b(Cytb)基因,利用DNA条形码技术鉴定鼠种。方法 2013-2014年在中哈边境阿拉山口地区采集鼠类样本,形态学鉴定完毕后提取鼠肝脏DNA,用PCR方法扩增线粒体COⅠ和Cytb基因,进行测序及Blast分析,使用Mega 6.0软件构建分子进化树。结果共采集鼠类样本4属7种382只,COⅠ和Cytb基因鉴定与形态学鉴定结果相符。结论 DNA条形码技术可对鼠类进行有效的鉴定,为口岸快速准确地识别宿主及监控外来医学媒介生物提供了科学依据。Objective To evaluate the cytochrome C oxidase subunit I （CO I ） and cytochrome b （Cytb） gene as the DNA bareodes for identifying the rodent species in the Alataw pass, China- Kazakhatan. Methods The genome DNA were extracted from rodent samples of 7 speeies collected from Alataw pass, China-Kazakhatan during the 2013-2014. The mitochondfial CO I and Cytb genes was amplified by PCR and sequenced. The phylogenetie trees were constructed by Mega 6.0 based on the nueleotide sequences of rodents published in the GenBank and the homology was analyzed. Results The 382 rodent samples of 7 species were clustered into 4 genera during the 2013-2014. The identification of CO I and Cytb gene results were 100% matched with the morphological analysis. Conclusion DNA bareoding can be used to effectively identify rodent species. DNA barcode technology provides a quick and accurate tool to identify the pathogen hosts and exotic medical vectors.

关 键 词：DNA条形码 中国-哈萨克斯坦边境 种类鉴定 细胞色素C氧化酶亚基Ⅰ 细胞色素B 

分 类 号：S443[农业科学—农业昆虫与害虫防治]