河北省116株鼠疫耶尔森菌差异片段基因分型及流行病学分析  

Genotyping of Yersinia pestis by different regions and its epidemiological characteristics in Hebei province

作　　者：王海峰[1] 刘合智[1] 白雪薇[1] 杨顺林[1] 周松[1] 胡乐乐[1] 刘溢洋[1] 李玉贵[1] 史献明[1] 

机构地区：[1]河北省鼠疫防治所检验科,河北张家口075000

出　　处：《中国媒介生物学及控制杂志》2016年第5期470-473,共4页Chinese Journal of Vector Biology and Control

基　　金：河北省2014年度医学科学研究重点课题计划(ZL20140119)~~

摘　　要：目的研究河北省鼠疫疫源地鼠疫耶尔森菌（鼠疫菌）菌株的基因型别和遗传特征。方法根据鼠疫基因组的22个差异区段和pMT1设计引物,对河北省鼠疫疫源地分离的116株菌株进行分析。结果116株鼠疫菌株均缺乏DFR01、DFR06、DFR07、DFR13、DFR15-18片段,参考DFR基因分型系统,河北省鼠疫疫源地的菌株为基因17型,主要分布于内蒙古自治区化德县、白旗、太仆寺旗相邻的康保县北部。结论河北省鼠疫菌菌株遗传稳定,仅有1个基因分型,疫情流行趋于平缓。Objective To study the genotype and epidemiological characteristics of Yersinia pestis in Hebei foci. Methods Primers were designed according to the confirmed 22 different segments （DFR）, to genotype the 116 Y. pestis DNA of Hebei province. Results All of the strains lack these DFR of 01, 06, 07, 13, 15-18, to compare the genetic typing system of DFR, Y. pestis from Hebei province belong to the 17 genotype, distributed in the northern Kangbao county neighboring Huade, Baiqi, and Taipusiqi in the Inner Mongolia Autonomous Region. Conclusion The K pestis is stable genetically in Hehei plague foci, and have only one genotype, so the epidemic tends to stabilize.

关 键 词：鼠疫耶尔森菌 差异区段 基因分型 

分 类 号：R254.8[医药卫生—中医内科学]