阿拉山口口岸地区多房棘球蚴线粒体NADH脱氢酶亚基1基因序列分析  

Analysis of the mitochondria gene NADH dehydrogenase 1 in Echinococcus multilocularis at Alataw pass, China-Kazakhstan

作　　者：尹小平[1] 王安东[1,2] 田延河[1] 梁臻[1] 巴特[1] 张江国[1] 

机构地区：[1]阿拉山口出入境检验检疫局,新疆博乐833418 [2]新疆石河子大学动物科技学院,新疆石河子832000

出　　处：《中国媒介生物学及控制杂志》2016年第5期481-483,共3页Chinese Journal of Vector Biology and Control

基　　金：国家质检总局科技计划项目(2016IK264)~~

摘　　要：目的分析阿拉山口口岸多房棘球蚴线粒体NADH脱氢酶亚基1(ND1)基因序列。方法使用ND1基因Cest引物对2014年捕获鼠类进行多房棘球蚴初筛,对阳性样品扩增ND1基因全长序列,利用Blast分析序列同源性、Mega 6.0软件构建分子遗传进化树,使用DNAStar软件分析其跨膜结构、信号肽、B细胞抗原表位等。结果阿拉山口口岸1.57%的野鼠感染多房棘球蚴,其线粒体ND1基因全长894 bp,编码297个氨基酸,与日本的多房棘球蚴AB018440和AB720065同源性最高,为100%。结论阿拉山口口岸地区野鼠存在多房棘球蚴感染,其序列信息为该地区多房棘球蚴的科学监控提供依据。Objective In order to analyze the NADH dehydrogenase subunit 1 （ND1） gene of Echinococcus multilocularis from the wild rodents at Alataw pass, China-Kazakhstan. Methods The ND1 genes of E. muhilocularis were amplified by PCR. The sequence was determined by Blast and phylogenetic analysis by Mega 6.0. The transmembrane domain （TM）, signal peptide and B cell epitope of the ND1 genes of E. multilocularis were predicted and analyzed by DNAStar. Results The positive rate of E. multilocularis was 1.57%. The results showed that the ND1 genes of E. multilocularis had a length of 894 bp, encoding 297 amino acids. E. multilocularis were 100% closely related to the E. multilocularis AB018440 and AB720065. Conclusion The E. multilocularis can infect wild rodents at Alataw pass. The sequence information of E. muhilocularis provides a basis for the scientific monitoring.

关 键 词：多房棘球蚴 线粒体 NADH脱氢酶亚基1 基因 阿拉山口口岸 

分 类 号：R383.3[医药卫生—医学寄生虫学]