肺结核患者血浆血小板源生长因子水平及其与载菌量的相关性  

The level of platelet-derived growth factor in patients with pulmonary tuberculosis and its correlation with bacteria load

作　　者：赵琰枫 余琴[3] 杨锡琴[2] 修冰水[2] 冯晓燕[2] 张贺秋[1,2] 

机构地区：[1]温州医科大学,浙江温州325035 [2]军事医学科学院基础医学研究所,北京100850 [3]北京市朝阳区疾病预防控制中心,北京100029

出　　处：《中国卫生检验杂志》2016年第19期2737-2739,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家高技术研究发展计划(2011AA02A113);军队重大项目(AWS15J006)

摘　　要：目的探讨血浆血小板源生长因子(PDGF-BB)水平在肺结核病患者与健康人之间是否具有显著差异,以及其水平与患者载菌量是否相关。方法收集134例初诊活动性肺结核病患者的EDTA抗凝血浆,并根据痰涂片及痰培养结果进行分组:涂片培养阳性组(S+/C+)55例,涂片阴性培养阳性组(S-/C+)43例,涂片培养阴性组(S-/C-)36例;健康对照组为健康体检40例。应用MILLIPLEXMAP Kit细胞因子试剂盒检测血浆中PDGF-BB的水平。结果结核病组血浆PDGF-BB水平显著高于健康对照组,差异有统计学意义(P<0.05);S+/C+组血浆PDGF-BB水平显著高于S-/C-组的测定值,差异有统计学意义(P<0.05);S+/C+组血浆PDGF-BB水平显著高于健康对照组,差异有统计学意义(P<0.01)。ROC曲线的AUC值为0.540,当临界值为271.4时,敏感度为28.6%,特异度为95.0%。结论 PDGF-BB水平与结核分枝杆菌感染及机体载菌量存在一定相关性,可能作为肺结核病患者临床辅助诊断的标志物之一,但是需要与其他细胞因子联合检测提高检测性能。Objective To investigate whether there is significant difference on the levels of platelet-derived growth factor（ PDGF-BB） between in patients with pulmonary tuberculosis and healthy people,and to analyze its correlation with bacteria load in patients with pulmonary tuberculosis. Methods EDTA anticoagulant plasma were collected from 134 newly diagnosed active pulmonary tuberculosis patients. Then these samples were divided into three groups according to the results of the sputum smear and sputum culture,including the sputum smear positive and culture positive group（ S ＋ / C ＋） containing 55 cases,sputum smear negative and culture positive group（ S-/ C ＋） containing 43 cases,and sputum smear negative and culture negative group（ S-/ C-） containing 36 cases. EDTA anticoagulant plasma were collected from 40 health people for physical examination as the normal control group. The levels of PDGF-BB in plasma were detected using MILLIPLEX MAP Kit. Results The level of plasma PDGF-BB in patients pulmonary tuberculosis group was higher than that in normal control group,with the difference statistically significant（ P 〈 0. 05）. Moreover,PDGF-BB levels in the S ＋ / C ＋ group was significantly higher than that in the S-/ C-group,with difference statistically significant（ P 〈 0. 05）; and that was also higher than in the control group,with the difference statistically significant（ P 〈 0. 01）. The AUC value of the ROC curve was 0. 540,when the critical value was 271. 4,the sensitivity was 28. 6% and the specificity was 95. 0%. Conclusion There is correlation between the level of PDGF-BB in plasma and Mycobacterium tuberculosis infection,as well as the bacteria load. The level of PDGF-BB in plasma may be a potential marker for clinical diagnosis of pulmonary tuberculosis. However,it should be needed to combine the PDGF-BB with other cytokines to improve the detection performance.

关 键 词：肺结核 血小板源生长因子 诊断 

分 类 号：R563.1[医药卫生—呼吸系统]