犬冠状动脉支架植入术后细胞凋亡及凋亡相关基因蛋白表达的变化  

Changes of apoptosis and expression of related apoptosis regulatory factors in neointima after canine coronary angioplasty

作　　者：聂晓敏[1] 李庚山[1] 江洪[1] 李晓艳[1] 蒋学俊[1] 王晓红[1] 杨新红[1] 漆曙辉[1] 许辉[1] 

机构地区：[1]武汉大学人民医院心内科

出　　处：《中国介入心脏病学杂志》2002年第3期155-157,共3页Chinese Journal of Interventional Cardiology

基　　金：湖北省九.五攻关项目 (962P1 1 0 1 )

摘　　要：目的　观察犬冠状动脉支架植入术后血管内膜凋亡及凋亡相关基因蛋白表达的动态变化。方法　 15只犬进行冠状动脉左回旋支支架植入术 ,前降支相应血管作为对照 ,分别于术后 1周、4周和 12周处死 ,进行病理学检查、TUNEL法和透射电镜检测凋亡细胞、PCNA和Bcl xl免疫组织化学染色以及Bcl xl蛋白质印迹杂交。结果　支架植入术后 1～ 12周内膜面积持续增加 ;正常血管未见凋亡细胞和PCNA阳性细胞 ,术后 1周内膜中凋亡细胞阳性率和PCNA阳性细胞率均达高峰 ,术后 4周和 12周不断降低 ,但凋亡细胞阳性率在各时间段均低于PCNA阳性细胞率 ;Bcl xl免疫组织化学染色和蛋白印迹杂交均显示正常对照血管偶见表达 ,术后 1周在内膜中表达增加 ,术后 4周在内膜中大量表达 ,并可维持至 12周。结论　细胞凋亡相对不足可能是支架植入术后再狭窄形成的一个原因 ,Bcl xl在内膜中高表达可能是造成细胞凋亡相对不足的因素之一。Objective To investigate the changes of apoptosis and expression of related apoptosis regulatory factors in neointima after canine coronary angioplasty Methods Stents were implanted in the left circumflex coronary artery in 15 canines Arteries were harvested at 1, 4, and 12 weeks after stenting Uninjured arteries were used as controls Apoptosis was demonstrated by the terminal uridine nick end labeling (TUNEL) method and transmission electron microscopy Proliferating cells were identified by immunostaining for proliferating cell nuclear antigen (PCNA) Expressions of Bcl xl proteins were detected by immunohistochemical method and Western blot Results Stents implantment induced intimal hyperplasia Apoptosis was not detected in control vessels Apoptotic cells and PCNA positive cells were identified at 1, 4, and 12 weeks with a peak at 1 week Profiles of apoptosis and cell proliferation after stenting were accordant in neointima, but cell proliferation rates were higher than cell apoptosis rates at all time points Expressions of Bcl-xl proteins were detected at 1 week, peaked at 4 weeks, and lasted till 12 weeks after stenting Conclusion Apoptosis may be an important determinant of in stent restenosis Bcl xl appears to play a critical role in regulating cell apoptosis

关 键 词：冠状动脉支架植入术 细胞凋亡 BCL-XL 术后 动物实验 基因表达 蛋白表达 

分 类 号：R654.2[医药卫生—外科学]