流动注射-分光光度法测定萝卜中过氧化物酶的活性  

FI-Spectrophotometric Determination of Peroxidase Activity of Radish

作　　者：郑巍[1] 李永生[1] 赵炀[1] 高秀峰[2] 

机构地区：[1]四川大学化学工程学院,成都610065 [2]四川大学华西基础医学与法医学院,成都610041

出　　处：《理化检验（化学分册）》2016年第10期1155-1159,共5页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用流动注射-分光光度法测定萝卜中过氧化物酶的活性。优化的试验条件如下：①反应温度为60℃;②载流为pH 5.5的0.05mol·L^-1磷酸二氢钠-磷酸氢二钠缓冲溶液;③采样体积为40μL;④反应盘管长度为200cm;⑤反应试剂为1.5mmol·L^-1过氧化氢,3.5mmol·L^-1愈创木酚的混合液。2-愈创木酚的表观摩尔吸光率为0.012 5L·μmol-1·cm^-1。辣根过氧化物酶的线性范围为454~7 265 U·L^-1,检出限（3s/k）为7 U·L^-1。方法的加标回收率在94.2%~107%之间。对500,2 500U·L^-1的辣根过氧化物酶标准溶液连续测定11次,测定值的相对标准偏差小于0.62%。FI-spectrophotometry was applied to the determination of activity of peroxidase in radish. The optimized conditions found were as follows： ① reaction temperature： 60 ℃; ② carrying solution： 0.05 mol·L^-1 NaH2PO4-Na2HPO4 buffer solution （pH 5.5）; ③ sampling volume： 40 μL; ④ length of reaction coil： 200 cm; ⑤ reaction reagent： mixture of 1.5 mmol·L^-1 H2O2 and 3.5 mmol·L^-1 2-guaiacol. Apparent molar absorptivity of 2-guaiacol found was 0.012 5 L·μmol-1·cm^-1. Linearity range of horse radish peroxidase was found between 454-7 265 U·L^-1 with detection limit （3s/k） of 7 U·L^-1. Results of test for recovery of this method were found in the range of 94.2%-107%. Precision of the method was tested at the concentration levels of 500, 2 500 U·L^-1 of horse radish peroxidase standard solution for 11 determination, values of RSD′s found were less than 0.62%.

关 键 词：分光光度法 流动注射 过氧化物酶 萝卜 

分 类 号：O657.32[理学—分析化学]