丛枝菌根真菌对丹参内源激素的影响  

Effects of arbuscular mycorrhizal fungi on endogenous hormones in Salvia miltiorrhiza

作　　者：周修腾[1,2] 王雪[1,3] 杨光[1] 陈敏[1] 李鹏英[1] 华国栋[3] 陈美兰[1] 郭姣[4,5] 

机构地区：[1]中国中医科学院中药资源中心道地药材国家重点实验室培育基地,北京100700 [2]广州中医药大学临床药理研究所,广东广州510405 [3]北京中医药大学东方医院药学部,北京100078 [4]广东省代谢性疾病中医药防治重点实验室,广东广州510006 [5]广东药科大学,广东广州510006

出　　处：《中国中药杂志》2016年第20期3761-3766,共6页China Journal of Chinese Materia Medica

基　　金：国家中医药管理局行业科研专项(201407005);中国中医科学院自主选题研究项目(Zz0708116)

摘　　要：该文旨在初步探索接种地表球囊霉（Glomus versiforme,GV）真菌不同时期丹参内源激素含量的动态变化,实验设定对照组和菌根组,测定接种GV菌剂30,60,75,90,105,130 d丹参根系侵染率,同时采用ELISA方法测定内源激素ABA,ZR,GA,IAA,Me JA含量的动态变化。结果表明,接种菌根90 d丹参根部侵染率为90%,基本达到平衡;地上部分鲜重和叶片数显著高于对照组,约为对照组的2.7,1.96倍;除ABA外,丹参接种菌剂75 d地上部分其他内源激素含量均显著低于对照组（P〈0.05）,分别约为对照组含量的63%~75%;接种菌剂90 d后地下部分内源激素含量均显著低于对照组（P〈0.05）,分别约为对照组含量的45%~81%;接种GV菌剂105 d后地下部分内源激素含量均显著高于对照组（P〈0.05）,分别高1.4~1.7倍;接种菌剂60,75,105 d地下部分ABA含量显著增加。因此,推论丛枝菌根真菌能促进丹参生长,且不同侵染时期影响丹参内源激素含量。To investigate the dynamic changes of endogenous hormones contents in Salvia miltiorrhiza after the inoculation of Glomus versiforme（ GV）. The contents of endogenous hormones of ABA,ZR,GA,IAA and Me JA by ELISA were measured. Infection rata of GV reached plateau of 90% at the 90^th d of inoculation; fresh weight of overground part and leaf number were significantly higher in GV group,with 2. 7 and 1. 96 fold than that of control; contents of all endogenous hormones apart from ABA in over- and under-ground part were markedly lower（ P〈0. 05） in GV group at the 75^th and 90^th d,respectively,with 63% to 75% and 45% to 81% of that in control,and were significantly higher（ P〈0. 05） in both over- and under-ground part in GV group at the 105^th d,with 1. 4 to 1. 7 fold higher than that of control; content of ABA in underground part increased significantly at 60^th,75^th and 105^th d. Arbuscular mycorrhizal fungi could promote the growth of S. miltiorrhiza,and affect the dynamic changes of endogenous hormones contents in different infection periods.

关 键 词：丛枝菌根真菌 丹参 内源激素 

分 类 号：S567.53[农业科学—中草药栽培]