一测多评法测定新疆紫草中主要萘醌类成分的含量  

Determination of five naphthaquinones in Arnebia euchroma by quantitative analysis multi-components with single-marker

作　　者：赵文文[1] 吴智敏[1] 吴霞[1,2] 赵海誉[3] 陈筱清[1,2] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]首都医科大学中医络病研究北京市重点实验室,北京100069 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2016年第20期3792-3797,共6页China Journal of Chinese Materia Medica

基　　金：北京高等学校高水平人才培养"实培计划"项目

摘　　要：以β,β'-二甲基丙烯酰阿卡宁为内参物,测定新疆紫草中乙酰紫草素、β-乙酰氧基异戊酰阿卡宁、异丁酰紫草素、α-甲基-正丁酰紫草素的含量,建立紫草中5种萘醌类成分的一测多评定量分析方法。采用高分辨LC-MS对含量测定的5个色谱峰进行鉴定。采用不同高效液相(HPLC)系统和不同色谱柱考察相对校正因子的耐用性,同时采用外标法与一测多评法对16批新疆紫草药材进行分析,并对含量测定结果进行比较。结果表明相对校正因子的耐用性良好,16批样品一测多评法与外标法所得结果无显著差异。所建立的含量测定方法可用于同时测定新疆紫草中5种萘醌类成分。This study is to determine five naphthaquinones（ acetylshikonin,β-acetoxyisovalerylalkannin,isobutylshikonin,β,β＇-dimethylacrylalkannin,α-methyl-n-butylshikonin） by quantitative analysis of multi-components with a single marker（ QAMS）. β,β＇-Dimethylacrylalkannin was selected as the internal reference substance,and the relative correlation factors（ RCFs） of acetylshikonin,β-acetoxyisovalerylalkannin,isobutylshikonin and α-methyl-n-butylshikonin were calculated. Then the ruggedness of relative correction factors was tested on different instruments and columns. Meanwhile,16 batches of Arnebia euchroma were analyzed by external standard method（ ESM） and QAMS,respectively. The peaks were identifited by LC-MS. The ruggedness of relative correction factors was good. And the analytical results calculated by ESM and QAMS showed no difference. The quantitative method established was feasible and suitable for the quality evaluation of A. euchroma.

关 键 词：新疆紫草 一测多评 相对校正因子 萘醌 LC-MS 

分 类 号：R284.1[医药卫生—中药学]