阿萨伊对虚热及虚寒证候模型的生物学表达特征影响  

Effect of Acaí(Euterpe oleracea) on biological expression characteristics of deficiency-heat and deficiency-cold rats

作　　者：王林元[1] 张建军[2] 王淳[2] 朱映黎[2] 王子晨[2] 贺成[3] 瞿研[1] 王莎[2] 

机构地区：[1]北京中医药大学中药学院,北京100029 [2]北京中医药大学基础医学院,北京100029 [3]北京中医药大学北京中医药研究院,北京100029

出　　处：《中国中药杂志》2016年第20期3833-3838,共6页China Journal of Chinese Materia Medica

基　　金：北京中医药大学自主课题(2015-JYB-XS002;2016-JYB-JSPY-013)

摘　　要：研究阿萨伊对虚热证、虚寒证大鼠生物学表达特征的影响。SD大鼠分为空白组;虚热模型组,虚热模型加黄柏组、加阿萨伊高、低剂量组;虚寒模型组,虚寒模型加肉桂组、加阿萨伊高、低剂量组;采用每日肌肉注射地塞米松磷酸钠、氢化可的松琥珀酸钠1次、连续21 d的方法制备大鼠虚热模型、虚寒模型;通过放免法检测环核苷酸(cAMP,cGMP),内分泌激素(T3,T4,r T3)指标变化;通过比色法检测代谢(TP,UA,TC,TG,ALB)指标变化。虚热阿萨伊高剂量组能降低血清中cAMP,cAMP/cGMP值(分别P<0.05,P<0.001);虚热阿萨伊高剂量组均能显著降低T3,T4,r T3值(分别P<0.01,P<0.001,P<0.05);虚热阿萨伊高剂量组均能显著降低TP,UA,TC,TG,ALB值(分别P<0.001,P<0.05,P<0.05,P<0.05,P<0.01);阿萨伊对虚寒模型作用不明显。阿萨伊同黄柏一致,表现出对虚热模型大鼠的调整作用;与肉桂相反,对虚寒模型大鼠的指标纠正不明显。该文从同类比较、异类反证角度验证了理论推导的阿萨伊中药寒凉药性。To study the effects of Acaí on biological expression characteristics in rats with deficiency-heat and deficiency-cold syndromes,SD rats were divided into blank group,deficiency-heat model group,deficiency-heat ＋ Phellodendri Chinensis Cortex group,deficiency-heat ＋ Acaí high dose and low dose groups,deficiency-cold model group,deficiency-cold ＋ Cinnamomi Cortex group,deficiency-cold ＋ Acaí high dose and low dose groups. The rats were treated with intramuscular injection of hydrocortisone（ 20 mg·kg^- 1）or dexamethasone sodium phosphate（ 0. 35 mg·kg- 1） for 21 days to set up deficiency-heat model and deficiency-cold models. The levels of cAMP,cGMP,T3,T4 and r T3 were detected by radioimmunoassay. The levels of TP,UA,TC,TG and ALB were detected by colorimetry. The level of cAMP,cAMP / cGMP in serum were reduced in Acaí high dose group（ P〈0. 05,P〈0. 001）. The levels of T3,T4 and r T3 were significantly reduced in the Acaí high dose group（ P〈0. 01,P〈0. 001,P〈0. 05）. The levels of TP,UA,TC,TG and ALB were significantly reduced in the Acaí high dose group（ P〈0. 001,P〈0. 05,P〈0. 05,P〈0. 05,P〈0. 01）.However,Acaí had no obvious effects on deficiency-cold models. Acaí showed the same effect with Phellodendri Chinensis Cortex in adjusting the levels of deficiency-heat rats; but unlike Cinnamomi Cortex,Acaí showed no obvious effects in adjusting the levels of deficiency-cold rats.

关 键 词：阿萨伊 虚热证 虚寒证 中药药性 生物学表达特征 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]