复方血栓通胶囊化学成分在比格犬眼球和肾脏内的分布研究  

Distribution of chemical compounds of Fufang Xueshuantong capsule in eyes and kidney of beagle dogs

作　　者：江美芳[1] 王勤辉[1] 庞欢欢[1] 田悦[1] 王晓烨[1] 孙璐[1] 高伟[1] 黄建梅[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2016年第20期3846-3851,共6页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2011ZX09201-201-22)

摘　　要：糖尿病视网膜病变(DR)和糖尿病肾病(DN)是最常见的糖尿病并发症,也是糖尿病致死致残的主要原因,临床报道显示复方血栓通胶囊对DR和DN均有较好的治疗作用,但复方血栓通胶囊中的化学成分在眼球和肾脏中的分布尚无报道,该研究主要探索复方血栓通胶囊中的化学成分在比格犬眼球和肾脏中的分布情况。比格犬连续灌胃给予复方血栓通胶囊3d后,取眼球和肾脏,运用高效液相色谱-高分辨液质联用技术LTQ-Orbitrap鉴定其中的化学成分,通过与对照品及相关文献中化学成分的精确相对分子质量、保留时间以及二级碎片信息比对,确认化合物。该研究在眼球和肾脏中分别鉴定出14,19个三七皂苷类成分。鉴定结果为阐释复方血栓通胶囊在治疗DR和DN的有效成分奠定了一定的物质基础。Diabetic retinopathy（ DR） and diabetic nephropathy（ DN） are the most common complications of diabetes,and the main causes of death and disability of diabetes. Clinical reports showed that Fufang Xueshuantong capsule（ FXT） had effective curative effect on DR and DN,but there was no report on the distribution of chemical compounds of FXT in beagle dog eyes and kidneys. In this study,FXT was given by gavage administration in Beagle dogs for 3 days,and then their eyeballs and kidneys were taken. The chemical compounds in beagle dog eyes and kidneys were detected by HPLC LTQ-Orbitrap technology. Furthermore,by comparing with the data from retrieving literature and references,the chemical compounds were identified by the accurate mass,retention time（ tR）,and MS / MS. Fourteen and 19 types of notoginsenosides were respectively identified in eyeballs and kidneys in this study,and these results could lay foundation for clarifying the effective ingredients of FXT in treatment of DR and DN.

关 键 词：复方血栓通胶囊 三七皂苷 眼球 肾脏 HPLC LTQ-Orbitrap 

分 类 号：R285.5[医药卫生—中药学]