溶藻菌A2对4种微藻的溶藻效果分析  

Specificity of algicidal activity against four species of microalgae by algicidal bacterium A2

作　　者：郗建云 曹煜成[2] 李卓佳[2] 文国樑[2] 徐武杰[2] 郑奇宏 李振成[2] 许云娜 

机构地区：[1]上海海洋大学水产与生命科学学院,上海201306 [2]中国水产科学研究院南海水产研究所,广东省渔业生态环境重点实验室,农业部南海渔业资源开发利用重点实验室,广东广州510300

出　　处：《南方水产科学》2016年第5期34-42,共9页South China Fisheries Science

基　　金：国家科技支撑计划项目(2011BAD13B10);现代农业(虾)产业技术体系建设专项资金(CARS-47);广东省海洋渔业科技与产业发展专项(A201401B03);广东省科技计划项目(2014B040404056);海南省自然科学基金项目(20163148);中央级科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2014TS21)

摘　　要：将溶藻菌菌株A2以初始浓度107CFU·m L-1接入到锥状斯氏藻(Scrippsiella trochoidea)、条纹小环藻(Cyclotella striata)、蛋白核小球藻(Chlorella pyrenoidosa)和四尾栅藻(Scenedesmus quadricauda)的纯培养与混合培养藻液中,研究A2对不同微藻的溶藻专一性,并通过16S r DNA序列分析对A2菌株进行菌株鉴定。结果表明,经16S r DNA鉴定,菌株A2属于海杆菌属(Marinobacter sp.);在微藻纯培养与混合培养环境下,A2皆可有效溶解甲藻类的锥状斯氏藻,对硅藻类的条纹小环藻具有一定的溶解效果,对绿藻类的四尾栅藻生长无影响,可以促进绿藻类蛋白核小球藻的生长。在多种微藻共存环境下,A2菌组锥状斯氏藻死亡率可高达98.29%和95.37%,蛋白核小球藻的浓度较对照组提高237.6%。结果表明,菌株A2具有良好的溶藻专一性,可作为研发养殖池塘甲藻调控剂的备选菌株。To investigate the specificity of algicidal activity against Scrippsiella trochoidea,Cyclotella striata,Chlorella pyrenoidosa and Scenedesmus quadricauda by bacterial Strain A2,we added Strain A2 to monoculture and mix-culture systems of these four microalage at concentration of 107CFU·m L^- 1,and analyzed the algicidal effects. The results show that the Strain A2 was identified as Marinobacter sp. by homology comparisons based on 16 S r DNA sequences. Strain A2 produced high lytic effect to S. trochoidea under monoculture and mix-culture systems,and had some algicidal effect to C. striata. The growth of S. quadricauda was not affected by Strain A2. However,Strain A2 could promote the growth of C. pyrenoidosa. Besides,98. 29% and 95. 37% of S. trochoidea were killed under the mix-culture systems of four microalage. The cell density of C. pyrenoidosa increased by 237. 6%,compared with the control under the mix-culture of S. trochoidea,C. striata and C. pyrenoidosa. It is indicated that Strain A2 has good specificity of algicidal activity to S. trochoidea,and can be potentially used to develop probiotics against dinoflagellate blooms in aquaculture ponds.

关 键 词：锥状斯氏藻 溶藻菌 专一性 

分 类 号：Q178.1[生物学—水生生物学]