鲑科鱼类线粒体全基因组序列结构特征及其系统发育信息分析  

Mitochondrial Genome Characteristics and Phylogenetic Analysis of Salmonidae Fishes Family

作　　者：李瑶瑶[1] 刘云国[1] 刘凌霄[1] 王敏强[1] LI Yao-yao LIU Yun-guo LIU Ling-xiao WANG Min-qiang(School of Life Sciences, Yantai University, Yantai 264005, Chin)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2016年第4期271-279,共9页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(31560719)

摘　　要：为了深入开展鲑科鱼类的线粒体全基因组序列的结构特征和系统发育信息的研究,利用生物信息学的方法分析了已获得的40种鲑科鱼类的线粒体全基因组序列.通过软件Clustal X进行对比,然后用软件MEGA5.05分析DNA的序列差异,并用邻接法生成系统进化树.结果发现：1）鲑科鱼类线粒体基因组的结构和基因排列顺序和其他硬骨鱼类的相同,其全长为16 526-16 997 bp.2）从系统进化树可以看出白鲑亚科（Coregonidaeinae）位于祖先的位置,是最早出现分化的一枝,鲑亚科（Salmoninae）和茴鱼亚科（Thymallinae）亲缘关系较近,互为姐妹群.3）在所有编码基因中,序列变异程度最大的是ND2基因（47.6%）,最小的是16S rRNA基因（11.0）;Kimura双参数遗传距离最大的是ND2基因（0.203）,最小的是12S rRNA基因（0.037）.40 Mitochondrial genomes of all of the 40 known Salmonidae fishes are analyzed using bioinformatics methods in order to study their characteristics of mitochondrial genome and phylogenetic relationship. Phylogenetic trees are generated using Neighbor-Joining method based on alignment and analysis of the DNA sequences using Clustal X and MEGA 5.05 softwares, respectively. The results are as follows： 1 ） Mitochondrial genome composition and gene order of the Salmonidae fishe family are consistent with other teleost fish species. The mitochondrial genome sizes are from 16 526 bp to 16 997 bp. 2） According to the phylogenetic trees, the earliest differentiation is the whitefish subfamily（Coregonidaeinae） which locates in the ancestor position. The salmon subfamily （Salmoni- nae） and the grayling subfamily（Thymallinae） are sister groups. 3 ）Among all the inverstigated genes, the highest sequence divergence is for ND2 gene （47.6%） , while the lowest one is for 16S rRNA gene （11.0）. The highest Kimura 2-Parameter genetic distance is for ND2 gene （0. 203 ）, while the lowest one is for 12S rRNA gene （0. 037）.

关 键 词：鲑科 线粒体基因组 结构特征 系统发育 

分 类 号：S917.4[农业科学—水产科学] Q959.4[生物学—动物学]