叶酸与Vit B_(12)联合应用对糖尿病大鼠心肌细胞凋亡的调控  

Regulation of myocardial cell apoptosis by folic acid combined with vitamin B_(12) in diabetic rats

作　　者：杨阳[1] 周大宇[2] 李强[3] 刘洋[1] 安静思[1] 

机构地区：[1]锦州市中心医院,辽宁锦州121000 [2]锦州市疾病预防控制中心 [3]辽宁医学院附属第一医院

出　　处：《实用预防医学》2016年第11期1330-1334,共5页Practical Preventive Medicine

基　　金：辽宁医学院青年科技启动基金(Y2012Z002);辽宁省大学生创新创业训练计划项目(201310160040)

摘　　要：目的检测2型糖尿病大鼠血液中一氧化氮(NO)、肌酸激酶(CK)、超氧化物歧化酶(SOD)及微量丙二醛(MDA)的变化情况和心肌组织的凋亡情况,探讨叶酸和维生素B_(12)干预对大鼠心肌的保护作用。方法六周龄SD大鼠60只,糖尿病造模成功后,随机分为对照组(A组)、模型组(B组)、叶酸和维生素B_(12)干预组(C组)。12周后心脏取血,检测各组NO、CK、SOD及MDA的水平;取大鼠心肌组织,Tunel法观察心肌组织的细胞凋亡情况;免疫组化法检测Caspase-3、Caspase-8的表达,并用计算机图像分析系统测平均灰密度值。结果 12周后心脏血液中,B组NO、SOD水平较A组明显下降,C组较B组明显增高(P<0.01);B组CK、MDA水平较A组明显增高,C组较B组明显下降(P<0.01);心肌组织中,三组的凋亡指数分别为(2.6357±0.5214)、(16.2859±0.6983)、(4.0410±0.3121);Caspase-3各组分别为(79.2471±2.3547)、(97.1530±4.9522)、(84.1549±3.0198);Caspase-8各组分别为(70.2596±3.2118)、(98.9322±5.0583)、(78.3299±3.5680)。B组与C组细胞凋亡指数、Caspase-3、Caspase-8的表达均高于A组(P<0.01),C组较B组明显下降(P<0.01)。结论 2型糖尿病大鼠血液中NO、SOD、CK、MDA的变化参与心肌细胞的凋亡过程,而叶酸和维生素B_(12)的干预可以有效缓解心肌损伤。Objective To detect the variation of blood nitric oxide （NO）, creatine kinase （CK）, superoxide dismutase （SOD） and malonyldialdeyde （MDA） and the apoptosis of myocardial tissues in type 2 diabetic rats, and to explore the protective effects of folic acid and vitamin B12 on myocardial tissues in rats. Methods Sixty SD rats aged 6 weeks were used to establish rat models of diabetes mellitus successfully, and then randomly divided into the control group （ group A）, the model group （group B） and folic acid and vitamin B12 intervention group （group C）. After 12 weeks, all the rats were sacrificed for assaying the levels of NO, CK, SOD and MDA in the blood drawn from hearts. Tunel method was used to observe myocardial cell apoptosis. Immunohistochemical method was employed to determine the expression of caspase-3 and caspase-8, and the average gray density values of caspase-3 and caspase-8 were measured by using computer image analysis system. Results After 12 weeks, the levels of blood NO and SOD were significantly lower in group B than in group A （P〈0.01）, but significantly higher in group C than in group B （P〈0.01）. The levels of CK and MDA were significantly higher in group B than in group A （P〈0.01）, but significantly lower in group C than in group B （P〈0.01）. The apoptotic indexes of myocardial cells in cardiac muscle tissues of the 3 groups were （2.6357±0.5214）,（16.2859±0.6983） and （4.0410±0.3121） respectively. The expression of Caspase-3 and Caspase-8 in the 3 groups was （79.2471±2.3547） and （70.2596±3.2118）, （97.1530±4.9522） and （98.9322±5.0583）, （84.1549±3.0198） and （78.3299±3.5680） respectively. The apoptotic indexes of myocardial cells and the expression of caspase-3 and caspase-8 were significantly higher in groups B and C than in group A （P〈0.01）, but significantly lower in group C than in group B （P〈0.01）. Conclusions The changes of blood NO, CK, SOD and MDA in myocardial tissues of type 2 diabeti

关 键 词：糖尿病 大鼠 叶酸 维生素B12 心肌组织 

分 类 号：R587[医药卫生—内分泌]