输入性三日疟原虫的实验室诊断  

Laboratory diagnosis of imported Plasmodium malariae infection

作　　者：徐明忠[1] 田斌[1,2] 张兵[1] 黄非凡 廖瑜[1] 申晓君[1] 文岚[1] 

机构地区：[1]长沙市疾病预防控制中心,湖南长沙410001 [2]中南大学湘雅医学院

出　　处：《实用预防医学》2016年第11期1391-1394,共4页Practical Preventive Medicine

基　　金：长沙市科技局2013年度指导性科技计划项目(K13ZD051-33)

摘　　要：目的分析输入性三日疟疾的实验室诊断,减少三日疟的误诊与漏诊。方法采用厚薄血膜涂片形态学检查、巢氏PCR对疟原虫18S rRNA基因进行扩增等方法确诊输入性三日疟疾病例。结果经过形态学和巢氏PCR检测发现首诊结果为未检出、检出未分型和诊断为恶性疟的7例患者感染三日疟原虫,有一例为间日疟原虫和三日疟原虫混合感染。结论在消除疟疾的地区,应当持续的进行疟原虫显微镜镜检技术的培训和分子生物学技术的推广应用,以满足现阶段输入性疟疾病例正确诊断和分型要求,尤其是罕见和少见虫种鉴定要求。Objective To analyze the laboratory diagnosis of cases of imported Plasmodium malariae infection so as to reduce its misdiagnosis and missed diagnosis. Methods The cases of imported Plasmodium malariae infection were confirmed by mor-phological test of thick and thin blood smears and nested polymerase chain reaction （PCR） malaria detection assay. Results The results of morphological test and nested PCR malaria detection assay showed that 7 cases of Plasmodium malariae infection which were initially diagnosed with non-Plasmodium infection, Plasmodium infection and Plasmodium falciparum infection were detected, including 1 case of mixed Plasmodium malariae and Plasmodium vivax infections. Conclusions The training about morphological diagnosis of Plasmodium species and the related molecular biology techniques should be continuously strengthened and promoted in malaria eliminated areas so as to meet the correct diagnosis and species identification of imported malaria cases at the present stage, especially in rare and seldom species identification.

关 键 词：三日疟原虫 巢氏PCR 混合感染 实验室诊断 

分 类 号：R446[医药卫生—诊断学]