补阳还五汤含药血清对人脑微血管内皮细胞增殖和迁移的影响  

Effect of Buyang Huanwu Decoction on Proliferation and Migration of Human Brain Microvascular Endothelial Cells

作　　者：叶淑静[1] 孙斯琪 李琳[3] 方燕[3] 杨琰[3] 储利胜[3] 

机构地区：[1]浙江中医药大学附属杭州第一医院中药房,杭州310006 [2]浙江中医药大学药学院,杭州310053 [3]浙江中医药大学基础医学院,杭州310053

出　　处：《浙江中西医结合杂志》2016年第10期887-889,F0003,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省自然科学基金项目(No.Y207771);浙江中医药大学基础医学院科技创新团队资助项目(No.YCIT2016-2)

摘　　要：目的观察不同浓度补阳还五汤(BYHWD)含药血清对人脑微血管内皮细胞(HBMECs)增殖和迁移影响。方法补阳还五汤灌胃SD大鼠后腹主动脉取血,制备含药血清;体外培养人脑微血管内皮细胞(HBMECs),随机分为空白组(正常大鼠血清)及补阳还五汤含药血清组,血清浓度分为5%、10%和20%3个浓度,共6组;四甲基偶氮唑蓝(MTT)法检测HBMECs增殖;细胞划痕法检测HBMECs迁移的相对距离。结果与空白组比较,5%、10%和20%含药血清均显著促进HBMECs增殖,差异有统计学意义(0.71±0.03比0.65±0.03、0.84±0.05比0.75±0.03、0.71±0.05比0.67±0.05,P<0.05);与10%含药血清组比较,5%含药血清组和20%含药血清组细胞增殖显著减少,差异有统计学意义(0.71±0.03、0.71±0.05比0.84±0.05,P<0.05)。与空白组比较,各含药血清组细胞迁移的相对距离均显著增加,差异有统计学意义(0.58±0.03比0.50±0.01、0.63±0.03比0.45±0.05、0.53±0.05比0.45±0.04,P<0.05);与10%含药血清组比较,5%含药血清组和20%含药血清组细胞迁移的相对距离显著减小,差异有统计学意义(0.58±0.03、0.53±0.05比0.63±0.03,P<0.05)。结论补阳还五汤含药血清可促进HBMECs增殖和迁移,10%含药血清作用最佳。Objective To investigate the effect of Buyang huanwu decoction（BYHWD） with different concentrations on the proliferation and migration of human brain microvascular endothelial cells（HBMECs）. Methods The abdominal aortic blood from SD rats treated with BYHWD was drawn to prepare drugontaining sera. HBMECs were cultured in vitro and randomly divided into blank groups （normal rat serum at 5%, 10% and 20% concentrations, respectively） and BYHWD drug-containing serum groups （at 5%, 10% and 20% concentrations, respectively）. The proliferation of HBMECs was determined by MTT, and the influence of migration was detected by cell scratch method. Results Compared with blank groups, drug-containing serum at 5%, 10% and 20% concentrations significantly promoted cell proliferation （0.71 0.03 vs 0.65 0.03, 0.84 0.05 vs 0.75 0.03, 0.71 0.05 vs 0.67 0.05, all P〈0.05）; compared with drug-containing group at 10% concentration, the proliferation of HBMECs was reduced with a significant difference in drug-containing group at 5% and 20% concentrations（0.71 0.03, 0.71 0.05 vs 0.84 0.05, P〈0.05）. The relative distance of HBMEC migration was longer in BYHWD groups than those in blank groups（5%： 0.58 0.03 vs 0.50 0.01, 10%： 0.63 0.03 vs 0.45 0.05, 20%： 0.53 0.05 vs 0.45 0.04; all P〈0.05）. A significant reduction in relative distance of HBMEC migration was found in drug-containing groups at 5% and 20% concentrations compared to at 10% concentration（0.58 0.03, 0.53 0.05 vs 0.63 0.03, P〈0.05）. Conclusion BYHWD can promote the proliferation and migration of HBMECs and the effect is maximal when the concentration of the drug-containing sera is 10%.

关 键 词：人脑微血管内皮细胞 血清 补阳还五汤 增殖 迁移 

分 类 号：R285[医药卫生—中药学]