蛙骨骼肌检测紫色甘薯醇提物抗疲劳效应研究  

Study on frog skeletal muscle test the anti-fatigue effect of alcohol extract of purple sweet potato

作　　者：熊斌[1,2] 陶小丹 王杨科[1] XIONG Bin TAO Xiao-dan WANG Yang-ke(School of Bioscience and Engineering, Shaanxi Sci-Tech University, Hanzhong 723000, China School of Economics and Law, Shaanxi Sci-Tech University, Hanzhong 723000, China Senior Middle School of Baihe County, Baihe 725800, China)

机构地区：[1]陕西理工大学生物科学与工程学院,陕西汉中723000 [2]陕西省白河县高级中学,陕西安康725800 [3]陕西理工大学经济与法学学院,陕西汉中723000

出　　处：《陕西理工学院学报（自然科学版）》2016年第5期69-73,79,共6页Journal of Shananxi University of Technology:Natural Science Edition

基　　金：陕西省农业科技创新与攻关项目(2015NY156);陕西省教育厅科研基金资助项目(13JS022)

摘　　要：研究紫色甘薯醇提物对蛙骨骼肌收缩舒张的影响,筛选出蛙骨骼肌收缩曲线共性因子作为骨骼肌研究抗疲劳指标,为蛙骨骼肌作为初步检测抗疲劳物方法提供依据。将蛙神经-腓肠肌标本连接到张力换能器,刺激神经干,用生理信号采集系统记录相应的曲线。统计骨骼肌收缩曲线上TFmax50、Tmax、△T、STI、DTI、DTI50、DTI90、+d T/dtmax、-d T/dtmax、t-d T/dtmax,并做因子分析。设计小鼠体内实验,对蛙骨骼肌检测紫色甘薯醇提物抗疲劳效应的可靠性进行验证。因子分析提取5个因子成分,累计方差贡献率达到90.22%,较好地代替原始10个指标来评价紫色甘薯醇提物对蛙骨骼抗疲劳效应。因子1(DTI50、DTI90、TFmax50)可初步认定为综合因子,主要表现的是骨骼肌舒张持续时间。DTI50、DTI90、TFmax50在蛙骨骼实验组与空白组之间达到了极显著性差异(P<0.01),表明紫色甘薯醇提物对蛙骨骼具有抗疲劳效应,与小鼠体内实验结论一致。因此,DTI50、DTI90、TFmax50能很好地反应紫色甘薯醇提物对蛙骨骼抗疲劳效应。To investigate extract effect of of purple sweet potato on frog muscle contraction and diastole and filtrate common factor in curve of frog muscle contraction and diastole foranti-fatigue,the most preliminary testing method of fatigue resistance was studied. Connecting the frog nerves-gastrocnemius muscle tension transducer,stimulating the nerve trunk,the curve of muscle contraction and diastole by physiological signal acquisition system. factor analysis is made by TFmax50,Tmax,△T,STI,DTI,DTI50,DTI90,＋ d T / dtmax,-d T / dtmax,t-d T/dtmax. According to the technical specification for inspection and evaluation of health food,mice experiments on anti-fatigue effect of alcohol extract of purple sweet potato is designed. Factor analysis extract 5 constituent factors,and the cumulative variance is 90. 22%,which is a better substitution for the original 10 indexes to evaluate the anti-fatigue effect of alcohol extract of purple sweet potato by frog skeletal muscle. Factor 1（ DTI50,DTI90,TFmax50） can be initially identified as comprehensive factors,mainly for showing the duration of frog muscle diastole. DTI50,DTI90,TFmax50 in frog skeletal muscle reached significant difference between experimental group and the blank group（ P〈0. 01）,suggesting that alcohol extract of purple sweet potato by frog skeletal muscle is of great anti-fatigue effect and in agreement with the experimental conclusion in mice. DTI50,DTI90,TFmax50 can reflect of the anti-fatigue effect of alcohol extract of purple sweet potato on frog skeletal muscle. The results showed no difference from the test on mice.

关 键 词：因子分析 蛙骨骼肌 紫色甘薯醇提物 抗疲劳 

分 类 号：Q955[生物学—动物学]