一种高通量水稻DNA提取方法及其在种子纯度检测中的应用  

A high throughput DNA extraction method and its application in rice seed purity identification

作　　者：刘毅[1] 赵洪阳 唐金娟[1] 王加红[1] 刘国兰[1] LIU Yi ZHAO Hong-yang TANG Jin-juan WANG Jia-hong LIU Guo-lan(Shanghai Agrobiological Gene Center, Shanghai 201106, China Shanghai Tiangu Biotechnology Co. , Ltd. , Shanghai 201203, China)

机构地区：[1]上海市农业生物基因中心,上海201106 [2]上海天谷生物科技股份有限公司,上海201203

出　　处：《上海农业学报》2016年第5期8-10,共3页Acta Agriculturae Shanghai

基　　金：国家高技术研究发展计划(863计划)项目(2012AA101102);(2014AA10A604)

摘　　要：以杂交节水抗旱稻‘旱优73'和‘旱优3号'及其亲本为材料,采取一种高通量96深孔板提取水稻DNA的方法进行杂交种子纯度鉴定。结果表明:该提取方法获得的DNA纯度高,能满足正常的SSR分析,利用引物RM208和RM336扩增的条带清晰可辨,可对‘旱优73'和‘旱优3号'杂交种子纯度进行准确鉴定。The purity of rice hybrid seed was identificated by a method of high throughput DNA extraction in rice with deep-well plate,taking the hybrid water-saving and drought resistance rice ＇ Hanyou 73 ＇ and＇ Hanyou 3＇ and their parents as materials.The results showed that the purity of DNA obtained by the method was high and could meet the normal SSR analysis.The amplified bands by primer RM208 and RM336 were clearly,and the purity of hybrid seeds of ＇ Hanyou 73 ＇ and ＇ Hanyou 3 ＇ could be accurately identificated by the method.

关 键 词：高通量 DNA提取 纯度鉴定 

分 类 号：Q503[生物学—生物化学]