非洲菊花托的体细胞胚发生及植株再生  

Somatic embryogenesis and plantlet regeneration from receptacles of Gerbera jamesonii

作　　者：殷丽青[1,2] 孙翊[1,2] 李水根[1,2] 张永春[1] 陆锦明 范晓芬[1] 朱天华 YIN Li-qing SUN Yi LI Shui-gen ZHANG Yong-chun LU Jin-ming FAN Xiao-fen ZHU Tian-hua(Forestry and Pomology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China Shanghai Jingxiang Agricultural Science and Technology Co. , Ltd. , Shanghai 201106, China Nursery Garden of Minhang District, Shanghai 201109, China)

机构地区：[1]上海市农业科学院林木果树研究所,上海201403 [2]上海景香农业科技有限公司,上海201106 [3]上海闵行区苗圃,上海201109

出　　处：《上海农业学报》2016年第5期58-61,共4页Acta Agriculturae Shanghai

基　　金：上海市科技兴农攻关项目[沪农科攻字(2014)第1-3号];闵行区科技项目(2015MH109)

摘　　要：以非洲菊花托为外植体进行体细胞胚发生及植株再生研究。结果表明:非洲菊体细胞胚发生的适宜培养基为MS+BA 8 mg/L+KT 0.5 mg/L+NAA 0.5 mg/L+PIC 0.2 mg/L+TDZ 0.03 mg/L;以外植体初始培养时,先进行弱光照(400 lx)培养8 d,再置于强光照(2 500 lx)培养为宜,此条件下,体细胞胚再生率可达51.9%。外植体诱导培养40 d后开始有体细胞胚发生,50 d左右可达到发生高峰,体细胞胚的发生、发育是不同步的。将非洲菊成熟子叶胚转接于分化培养基:改良MS+KT 0.2 mg/L+IAA 0.2 mg/L,成苗率达100%。Receptacles were obtained as explants for the somatic embryo induction and plantlet regeneration of Gerbera jamesonii.The results showed that the optimized culture medium for somatic embryo induction was MS ＋ BA 8 mg/L ＋ KT 0.5 mg/L ＋ NAA 0.5 mg/L ＋ PIC 0.2 mg/L ＋ TDZ 0.03 mg/L.After cultured 8 days under weak light（400 lx）,the explants expected to produce somatic embryos needed to be transferred to strong light（2 500 lx）.Under this condition of light treatment,the induction rate of somatic embryos was up to 51.9%.After explants induction,somatic embryos were observed beginning at 40 days and peaking at 50 days.The production and development of somatic embryos were asynchronous.Cotyledon embryos of G.jamesonii were transferred to differentiation medium which was improved MS medium supplied with 0.2 mg/L KT and 0.2 mg/L IA A,and the seedling rate reached 100%.

关 键 词：非洲菊 体细胞胚 植株再生 花托 

分 类 号：S682.1[农业科学—观赏园艺] S603.6[农业科学—园艺学]