超高效液相色谱-串联质谱法测定黄芪中黄芪甲苷的含量  

Determination of astragaloside Ⅳ in Radix astragali by ultra high performance luquid chromatography-tandem mass spectrometry

作　　者：夏义平[1] 李建平[1] 党亚敏[1] 渠志华[1] 王辰[1] 

机构地区：[1]天津市疾病预防控制中心,天津300011

出　　处：《中国食品卫生杂志》2016年第5期624-627,共4页Chinese Journal of Food Hygiene

基　　金：天津市卫生计生委科技基金(2014KY21)

摘　　要：目的建立超高效液相色谱-串联质谱法测定黄芪中黄芪甲苷的含量。方法黄芪经甲醇-氨水（3：1,V/V）超声提取30 min,采用多反应监测（MRM）模式,以m/z 785.4/143.108和m/z 785.4/473.391为定性离子对,m/z 785.4/143.108为定量离子对,外标法测定黄芪甲苷。结果黄芪甲苷在60.0-12 000 ng/ml浓度范围内有良好线性关系（r=0.999 3）,平均回收率为91.7%,最低检测限为68.2μg/kg。结论该方法具有简便、快速和灵敏度高的特点,可用于黄芪中黄芪甲苷的测定。Objective To establish a method for determination of astragaloside Ⅳ in Radix astragali by UPLC-MS / MS.Methods Radix astragali was extracted with methanol-ammonia（ 3 ： 1,V / V） for 30 min by ultrasonic,the astragaloside Ⅳ was monitored via the ESI＋ ionization model and quantified by multiple reaction monitoring（ MRM）,using m / z 785. 4 /143. 108 and m / z 785. 4 /473. 391 as qualitative ion pair and m / z 785. 4 /143. 108 as quantitative ion pair. Results The calibration curve of astragaloside Ⅳ was in good linearity over the ranges of 60. 0-12 000 ng / ml（ r = 0. 999 3）,the average recovery was 91. 7%,and the detection limit was 68. 2 μg / kg. Conclusion This method was accurate,rapid,easy to operate,and the methodology validation showed that it was suitable for the determination of astragaloside Ⅳ in Radix astragali.

关 键 词：黄芪甲苷 超高效液相色谱-串联质谱法 多反应监测 功效成分 定量分析 黄芪 检测方法 

分 类 号：R284.1[医药卫生—中药学]