两种培养基间雨生红球藻细胞生长分化差异及磷的作用  

Effects of Phosphorus on H. pluvialis Cell Propagation and Differentiation in Two Medium

作　　者：徐晓莹[1,2] 程天佑[1,2] 陈林[1] 张维[1] 刘天中[1] XU Xiao-ying CHENG Tian-you CHEN Lin ZHANG Wei LIU Tian-zhong(Key Lab. Biofuels, Qingdao lnstitute of Bioenergy and Bioprocess Technology, CAS, Qingdao, Shandong 266101, China University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院青岛生物能源与过程研究所生物燃料重点实验室,山东青岛266101 [2]中国科学院大学,北京100049

出　　处：《过程工程学报》2016年第5期840-848,共9页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金中泰合作研究基金资助项目(编号:51561145015)

摘　　要：基于雨生红球藻游动细胞和不动细胞间Triton X-100敏感性的差异,建立了一种快速监测培养过程中细胞分化的指标,对比了雨生红球藻在BG11和BBM两种培养基中的细胞生长与分化差异.结果表明,在BBM培养基中培养8 d,雨生红球藻的游动细胞比例始终较高,而在BG11中早期不动孢子囊释放的游动孢子则快速成熟并转变成不动细胞.这主要是由于BBM中游动细胞通过有丝分裂或孢子囊两种模式维持了其继续分裂与增殖的能力,而BG11中这些繁殖形式缺失的主要原因在于其较低的磷浓度,培养基中适当的高磷含量对保持雨生红球藻培养中期游动细胞的持续繁殖非常关键.A novel method to fast characterize cell differentiation ofHaematococcus pluvialis SCCAP k-0084 by cell osmotic sensitivity of motile cells and non-motile cells on Triton X-100 was proposed and then cell propagation and differentiation of H. pluvialis in two fresh medium BGll and BBM were investigated. It was found that BBM medium kept a high percentage of motile cells in the first 8 d cultivation. In contrast, the released motile cells gradually transformed to non-motile cells. The cell proliferation in BBM medium is contributed by both mitosis and the formation of sporangium of motile cells. It is proved that such differences of cell propagation and differentiation are produced by that the low phosphorus concentration in BGll, indicating the importance of appropriate phosphorus concentration in medium to maintain the continuous division of motile cells.

关 键 词：雨生红球藻 繁殖方式 游动细胞 不动细胞 孢子囊 

分 类 号：Q819[生物学—生物工程]