纳米山药多糖结肠靶向胶囊对急性肝衰竭模型大鼠肠道菌群影响研究  

Research of Nano CYP Colon-Specific Capsule Influence the Rats Intestinal Flora on Acute Liver Failure

作　　者：高辛[1] 马淑霞[2] 陈杨[1] 李雪欣[1] 张灵芝[1] 石亿心 王丹[1] 赵文琦[1] 翟美芳 许月明[1] 于莲[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《时珍国医国药》2016年第9期2109-2112,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81274101)

摘　　要：目的评价研究纳米山药多糖对急性肝衰竭模型大鼠肠道菌群的影响。方法以硫代乙酰胺(TAA)腹腔注射造成大鼠肝衰竭并伴有肠道菌群失调模型,采用平板活菌计数法,检测造模前后大鼠的肠道菌落数量变化,判定模型是否成功,将大鼠随机分成正常对照组、纳米山药多糖组、丽珠肠乐组、自然恢复组。菌落计数法检测各组动物菌群数量,菌群易位,测定谷丙转氨酶(ALT)、总胆红素(TBIL)和肝脏指数。结果模型组与正常组比,双歧杆菌、乳酸杆菌数量下降,肠球菌、肠杆菌、类杆菌数量明显并出现菌群易位,肠道菌群失调模型建立。纳米山药多糖组与自然恢复组比可以显著抑制TAA引起的ALT、TBIL活性的升高,并降低大鼠肝脏指数,显著改善菌群失调和细菌易位情况。结论纳米山药多糖有改善急性肝衰竭模型大鼠菌群失调和细菌易位作用。Objective To evaluate and research the effects of nano yam polysaccharide on acute liver failure rats intestinal flora.Methods TAA causes liver failure in rats and accompanied by intestinal flora imbalance model,investigating the protection of it on acute hepatic failure model in rats induced by TAA. Using tablet live bacteria counting method,detecting the number change of rats intestinal bacterial colonies before and after building model to determine whether the model is successful or not. Rats were randomly divided into normal control group,nano CYP group,Lizhuchangle group,natural recovery group,detecting each number of animal bacteria,the bacteria translocation by colony counting method,detecting ALT,TBIL and liver index. Results The intestinal flora model was successfully established which determined by the number of bifidobacterium and lactobacillus were significantly decreased,the number of E. coli,enterococcus and bacteroides were increased significantly and occured bacterial translocation. Nano yam polysaccharide can significantly inhibit the increase of activity of TAA induced ALT,TBIL,and reduce the liver index,improve dysbacteriosis and bacterial translocation. Conclusion The experiment discovered for the first time,nano yam polysaccharide on improving the model of acute liver failure rat microflora disorders and bacterial translocation.

关 键 词：纳米山药多糖 硫代乙酰胺 急性肝衰竭 菌群失调 细菌易位 

分 类 号：R285.5[医药卫生—中药学]