Box-Behnken效应面法结合微遗传算法优化夏枯草的提取工艺  

Optimization of Extraction Process of Prunellae Spica by Box-Behnken Response Surface Methodology and Micro-genetic Algorithm

作　　者：曲永胜[1] 解盈盈 郭威[1,2] 周倩[1,2] 王亮[1,2] QU Yong-sheng XIE Ying-ying GUO Wei ZHOU Qian WANG Liang(Shandong Academy of Chinese Medicine, Ji＇nan 250014, China College of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji＇nan 250355, China)

机构地区：[1]山东省中医药研究院,济南250014 [2]山东中医药大学药学院,济南250355

出　　处：《中国实验方剂学杂志》2016年第20期14-17,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山东省中医药科技发展计划项目(2015-167)

摘　　要：目的:优化夏枯草中迷迭香酸的超声提取工艺,确定工艺参数的最优范围。方法:以乙醇体积分数、液固比、提取时间为考察因素,迷迭香酸提取率为评价指标,采用Box-Behnken效应面法进行试验设计并建立数学模型,采用微遗传算法对该模型进行优化,得到提取工艺参数最优范围。结果:夏枯草中迷迭香酸的最佳提取工艺为超声功率200 W,超声频率53k Hz,乙醇体积分数(50.03±1.21)%,液固比(48.28±2.27)m L·g^(-1),提取时间(47.34±1.43)min。迷迭香酸提取率0.301%。结论:Box-Behnken效应面法和微遗传算法联用时优化的迷迭香酸工艺提取率高,能得到具有浮动范围且不影响提取率、容易在生产中实现的工艺参数,可为中药提取工艺的研究提供参考。Objective： To optimize ultrasonic extraction process ranges of rosmarinic acid from Prunellae Spica. Method： Taking ethanol concentration, liquid-solid ratio and extraction time as factors, yield of rosmarinic acid as index,mathematical model was established by Box-Behnken response surface methodology. The model was optimized by micro-genetic algorithm in order to obtain the optimum ranges of extraction process parameters. Result： Optimal conditions of extraction process were as following： ultrasonic power of 200 W,ultrasonic frequency of 53 k Hz,ethanol concentration of（ 50. 03 ± 1. 21） %,liquid-solid ratio of（ 48. 28 ± 2. 27）m L·g^-1,extraction time of（ 47. 34 ± 1. 43） min. Yield of rosmarinic acid was 0. 301%. Conclusion： This optimized process has a high yield of rosmarinic acid,it can obtain a floated wide of process parameters.

关 键 词：夏枯草 工艺参数 最优范围 微遗传算法 迷迭香酸 超声提取法 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]