基于UPLC-Q-TOF-MS^E分析芍枣胶囊在大鼠体内的代谢产物  

Analysis on in Vivo Metabolites of Shaozao Capsules in Rats by UPLC-Q-TOF-MS^E

作　　者：王羽凝[1] 穆丽华[1] 许璐[1] 魏沛沛 王茹[1] 刘屏[1] WANG Yu-ning MU Li-hua XU Lu WEI Pei-pei WANG Ru LIU Ping(Chinese PLA General Hospital, Beijing 100853, China Bengbu Medical College, Bengbu 233003, China)

机构地区：[1]中国人民解放军总医院,北京100853 [2]蚌埠医学院,安徽蚌埠233003

出　　处：《中国实验方剂学杂志》2016年第20期77-81,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：军队"十二五"中医药推广研发项目(10ZYZ141)

摘　　要：目的:研究芍枣胶囊在抑郁模型大鼠体内的代谢产物,并归纳和总结其代谢途径。方法:大鼠灌胃芍枣胶囊提取液后,收集血液、尿液、粪便和全脑并进行预处理,采用UPLC-Q-TOF-MS^E分析,流动相0.1%甲酸-乙腈梯度洗脱,流速0.6m L·min^(-1),柱温45℃,在负离子模式下获得MS/MS^E质谱信息,通过Metabolynx软件处理后对芍枣胶囊中活性化合物在血浆、尿液、粪便和全脑中的代谢产物进行推测。结果:大鼠体内共鉴定得到代谢产物7个,其中芍药苷的2个,斯皮诺素的3个,3,6'-二芥子酰基蔗糖的2个。结论:芍枣胶囊中活性化合物芍药苷,斯皮诺素和3,6'-二芥子酰基蔗糖主要代谢产物存在于血液、尿液和粪便中,代谢途径主要有脱糖水解、甲基化、脱羰基等。Objective： To analyze three bioactive compounds from Shaozao capsules and their metabolites in depression model rats,and summarize their metabolic pathways. Method： After oral administration of Shaozao capsules extract,rat plasma,urine,feces and brain were collected,these samples were analyzed by UPLC-Q-TOF-MS^E,mobile phase was acetonitrile-0. 1% formic acid for gradient elution,flow rate was 0. 6 m L·min^-1,metabolites of bioactive compounds were processed with Metabolynx software. Result： According to the mass information,7 metabolites were discovered,including 3 metabolites of peoniflorin,2 metabolites of spinosin and 2 metabolites of 3,6＇-disinapoyl sucrose. Conclusion： The metabolites mainly exist in rat plasma,urine and feces. The metabolic reactions of these active compounds form Shaozao capsules are methylation,decarbonylation and hydrolysis-desugar,et al.

关 键 词：芍枣胶囊 芍药苷 斯皮诺素 3 6'-二芥子酰基蔗糖 代谢产物 

分 类 号：R969.1[医药卫生—药理学] R945[医药卫生—药学]