白芍总苷在佐剂性关节炎大鼠与正常大鼠体内的药动学差异  

Pharmacokinetic Differences of Total Glucosides from Paeoniae Radix Alba in Rats with Adjuvant Arthritis and Normal Rats

作　　者：袁秀泽 邹盛勤[1] 王晓刚[1] 易剑峰[1] YUAN Xiu-ze ZOU Sheng-qin WANG Xiao-gang YI Jian-feng(College of Chemical and Biological Engineering, Yichun Uninversity, Yichun 336000, Chin)

机构地区：[1]宜春学院化学与生物工程学院,江西宜春336000

出　　处：《中国实验方剂学杂志》2016年第20期86-89,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81060355)

摘　　要：目的:比较白芍总苷在佐剂性关节炎大鼠与正常大鼠体内药动学参数的差异,为临床合理用药提供参考。方法:采用大鼠右后足垫皮下注射完全弗氏佐剂建立大鼠佐剂性关节炎模型,应用RP-HPLC测定模型大鼠和正常大鼠灌胃给予白芍总苷后不同时间点的芍药苷的血药浓度,运用PKSolver V2.0软件计算药动学参数。结果:高、中、低剂量(1.80,0.90,0.47 g·kg^(-1))给药时,模型组大鼠体内芍药苷的药峰浓度(C_(max))分别为(7.93±1.09),(4.81±1.06),(1.02±0.82)mg·L^(-1),达峰时间(T_(max))均为180 min;在正常大鼠体内,芍药苷的半衰期(t_(1/2))分别为(215.63±5.26),(213.16±4.18),(208.55±4.94)min;C_(max)分别为(52.39±2.49),(24.52±1.69),(5.79±0.52)mg·L^(-1),AUC_(0-t)分别为(12 564.08±467.37),(5 839.10±380.86),(1 439.95±144.39)mg·L^(-1)·min;T_(max)均为150 min。结论:在类风湿性关节炎状态下芍药苷的吸收速率减缓,C_(max),AUC_(0-t)和AUC_(0-∞)均显著减小;类风湿性关节炎能影响白芍总苷在大鼠体内的药动学行为。Objective： To compare the pharmacokinetics of total glucosides from Paeoniae Radix Alba in rats with adjuvant arthritis and normal rats. Method： Adjuvant arthritis rat model was established by a single intradermal injection of complete Freund＇ s adjuvant（ CFA） into the rat right footpad. The plasma concentrations of paeoniflorin at different time after intragastric administration of total glucosides from Paeoniae Radix Alba were determined by RP-HPLC. The pharmacokinetic parameters were estimated by PKSolver V2. 0 software. Result：when administration of high,medium and low dose（ 1. 80,0. 90,0. 47 g·kg^-1）,Cmaxof paeoniflorin in model group rats were（ 7. 93 ± 1. 09）,（ 4. 81 ± 1. 06）,（ 1. 02 ± 0. 82） mg·L^-1,respectively; Tmaxwere 180 min. In the normal group,t1/2 of paeoniflorin were（ 215. 63 ± 5. 26）,（ 213. 16 ± 4. 18）,（ 208. 55 ± 4. 94） min; Cmax were（ 52. 39 ± 2. 49）,（ 24. 52 ± 1. 69）,（ 5. 79 ± 0. 52） mg·L^-1; AUC0-t were（ 12 564. 08 ± 467. 37）,（ 5 839. 10 ± 380. 86）,（ 1 439. 95 ± 144. 39） mg·L^-1·min,respectively; Tmaxwere 150 min. Conclusion：Absorption of paeoniflorin is slow under the state of rheumatoid arthritis,Cmax,AUC0-t and AUC0-∞ are significantly decreased. Rheumatoid arthritis can affect the pharmacokinetic behaviour of total glucosides from Paeoniae Radix Alba in vivo.

关 键 词：白芍总苷 佐剂性关节炎 芍药苷 完全弗氏佐剂 药代动力学 类风湿性关节炎 

分 类 号：R969.1[医药卫生—药理学] R284.1[医药卫生—药学]