复方丹参滴丸干预冠心病血瘀证凋亡相关基因临床分析  

Effect of Compound Danshen Dropping Pills in Interfering Apoptosis-related Genes of Coronary Heart Disease with Blood Stasis Syndrome

作　　者：杨戈[1] 姜燕[2] 李军[1] 邢雁伟[1] 李小强[2] YANG Ge JIANG Yan LI Jun XING Yan-wei LI Xiao-qiang(Guang＇ anmen Hospital, China Academy of Chinese Medicine Silences, Beijing 100053, China Tianjin Tasly Group, Tianjin 300402, China)

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]天士力制药集团股份有限公司生物研究中心,天津300402

出　　处：《中国实验方剂学杂志》2016年第20期153-157,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30672765);北京市自然科学基金项目(7062058);博士后科学基金项目(20060390587)

摘　　要：目的:研究复方丹参滴丸干预冠心病血瘀证凋亡相关基因,寻找复方丹参滴丸干预CHD血瘀证的有效靶点。方法:通过中西医结合病证临床研究,用逆转录-聚合酶链反应(RT-PCR)方法,观察常规治疗对照组治疗前后和复方丹参滴丸治疗组治疗前后凋亡相关基因的变化。结果:治疗组治疗后B淋巴细胞瘤-2(Bcl-2)Associated Transcription Factor1(BCLF1)mRNA表达显著降低(P<0.01),凋亡相关基因(Bcl-2 assaciated X protein,Bax)mRNA表达有降低趋势,两组治疗前后Bcl-2和凋亡信号转导分子(Cysteine asparate specific protease-3,Caspase-3)mRNA表达无明显差异。结论:复方丹参滴丸治疗后冠心病血瘀证患者相关基因凋亡相关转录因子BCLF1 mRNA表达显著减弱,凋亡相关基因Bax mRNA表达受到抑制,复方丹参滴丸可能通过调控细胞凋亡相关基因的表达,对冠心病血瘀证患者发挥治疗保护作用。Objective： In previous studies,we had screened and verified the relevant genes of coronary heart disease（ CHD） with syndrome of blood stasis（ SBS）. Relevant genes of CHD with SBS were studied to reveal the pathobiology,and discover effective targets of DSP（ compound Danshen dropping pills） in interfering CHD with SBS. Method： Reverse transcription-PCR was used to study the effect of DSP in interfering relevant genes and its clinic effect on CHD with SBS. Result： After treatment with DSP,the mRNA expression of BCLF1（ Bcl-2 associated transcription factor 1） decreased obviously（ P〈0. 01）,and the mRNA expression of Bax（ Bcl-2 assaciated X protein） showed a downward trend. But the mRNA expressions of Bcl-2（ B cell lymphoma/lewkmia-2） and Caspase-3（ cysteine asparate specific protease-3） had no difference before and after the treatment.Conclusion： The study proves that DSP can significantly reduce the mRNA expression of BCLF1 of relevant genes of CHD with SBS,and inhibit the mRNA expression of Bax. It treats CHD with SBS by regulating expressions of apoptosis-related genes of coronary heart disease with blood stasis syndrome.

关 键 词：冠心病 血瘀证 凋亡 相关基因 复方丹参滴丸 

分 类 号：R287[医药卫生—中药学]