高效液相色谱-荧光法同时测定人体尿液中的酪氨酸和色氨酸  

Simultaneous Determination of Tyrosine and Tryptophanhuman in Human Urine by HPLC-FLD

作　　者：杨永丽[1] 崔盼盼[1] 谭婷[2] 刘翻[2] 郭岚[2] 万益群[1,2] 

机构地区：[1]南昌大学化学学院,江西南昌330031 [2]南昌大学分析测试中心,江西南昌330047

出　　处：《分析科学学报》2016年第5期634-638,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21465017)

摘　　要：建立了高效液相色谱-荧光检测法同时测定人体尿液中的酪氨酸（Tyr）和色氨酸（Typ）。采用Diamonsil C18柱（250×4.6mm,5μm）,以水-乙腈（80∶20,V/V）为流动相,流速1.0mL/min,荧光检测波长为λex=260nm、λ（em）=340nm。Tyr和Typ的含量分别在0.10-6.0μg/mL及0.050-6.0μg/mL范围内与色谱峰面积呈良好的线性关系（r=0.9993）,检测限分别为0.05μg/mL和0.02μg/mL。在三个添加水平的回收中,两种氨基酸的平均回收率在86.3%-122.3%之间,相对标准偏差（RSD）小于8.51%。将所建立的方法应用于健康人、普通病人和癌症患者晨尿检测,结果发现：癌症患者尿样中色氨酸的水平与健康人和普通病人相比偏低。The method for the determination of tyrosine and tryptophan in human urine by HPLC-FLD was presented.The mobile phase was H2O-CH3CN（80∶20,V/V）,the flow rate was 1.0 mL/min,the excitation and emission wavelengths were 260 nm and 340 nm,respectively.The content of tyrosine in0.10-0.10μg/mL range had a good linear relationship with the chromatographic peak area,as well as tryptophan with the content range in 0.050-6.0μg/mL.The linear regression equations were y=12.219x＋0.6694（r=0.9991）and y=83.612x＋6.7351（r=0.9993）.The limits of detection were 0.05μg/mL and 0.02μg/mL.The average recoveries were in the range of 86.3%-122.3%,with relative standard deviations equal to or lower than 8.5%.The concentrations of tyrosine and tryptophan in urines were detected and compared between controls and cancer patients.The concentration of tryptophan in cancer groups was lower than that in the control groups.

关 键 词：酪氨酸 色氨酸 尿液 高效液相色谱-荧光法 

分 类 号：O657.72[理学—分析化学]