L-半胱氨酸/纳米金/DNA/壳聚糖修饰金电极测定布洛芬  

Electrochemical Detection of Ibuprofen on L-Cysteine/Au colloid/DNA/Chitosan Modified Gold Electrode

作　　者：李玲[1] 王娟[1] 刘计敏 张燕[1] 郑晶[1] 郭满栋[1] 

机构地区：[1]山西师范大学化学与材料科学学院,山西临汾041004

出　　处：《分析科学学报》2016年第5期644-648,共5页Journal of Analytical Science

基　　金：山西省自然科学基金(No.20001057)

摘　　要：用电沉积和滴涂的方法依次将L-半胱氨酸、纳米金、DNA、壳聚糖修饰在金电极表面,制备了稳定性、响应性良好的修饰电极(L-Cys/Au colloid/DNA/CS/Au/CME)。考察了影响实验结果的关键因素,确定了最佳实验条件,进一步采用循环伏安法和差分脉冲伏安法研究了布洛芬在L-Cys/Au colloid/DNA/CS/Au/CME上的电化学行为。在最佳实验条件下,当布洛芬浓度在1.0×10^(-7)~1.0×10^(-4) mol/L范围内时相应的氧化还原峰电流与浓度呈现良好的线性关系,检出限(S/N=3)达2.3×10^(-8) mol/L。结果表明,该修饰电极对布洛芬有良好的响应性能,据此建立了一种测定布洛芬的新方法。A modified gold electrode with good stablity and reproducibility was fabricated by electrodeposition and drop-coating method,which was denoted as L-cysteine/Au colloid/DNA/chitosan（L-Cys/Au colloid/DNA/CS/Au/CME）.The key factors affecting the experimental results were analyzed and the optimal experimental conditions were determined.Different electrochemical methods such as cyclic voltammetry,differential pulse voltammetry were used to investigate the electrochemical behaviors of Ibuprofen on L-Cys/Au colloid/DNA/CS/Au/CME along with the electrochemical parameters calculated.Under the optimum condition,the concentration of Ibuprofen showed good linear relationships with the reduction peak current of Ibuprofen in the range of 1.0×10^-7-1.0×10^-4 mol/L with the detection limit of 2.3×10^-8 mol/L（3σ）.The modified electrode was successfully applied for the determination of Ibuprofen in real samples with satisfactory results.

关 键 词：DNA 纳米金 L-半胱氨酸 布洛芬 

分 类 号：O657.1[理学—分析化学]