大黄虫丸加味对免疫性肝纤维化大鼠肝组织PDGF及PDGFR的影响  

Influence of variation on Dahuang Zhechong pill on PDGF protein and PDGFR protein in liver of rats with immune hepatic fibrosis

作　　者：王金光[1] 车念聪[1] 李文新[1] 杜宇琼[1] 司远[1] 刘晔[1] 赵晖[1] 

机构地区：[1]首都医科大学中医药学院中医临床基础学系

出　　处：《环球中医药》2016年第10期1190-1193,共4页Global Traditional Chinese Medicine

基　　金：2015年度首都中医药研究专项(15ZY01);2015年度首都医科大学校自然基金(2015ZR25)

摘　　要：目的观察大黄虫丸配伍黄芪、水红花子汤剂对免疫性肝纤维化大鼠肝组织血小板源性生长因子(platelet derived growth factor,PDGF)及血小板源性生长因子受体(platelet derived growth factor receptor,PDGFR)的影响。方法将SD大鼠随机分为正常组、模型组、中药组和秋水仙碱组。除了正常组外的3组均建立猪血清腹腔注射诱导的免疫性肝纤维化模型。灌胃治疗6周后,取大鼠肝脏进行病理HE染色及通过碱水解法检测羟脯氨酸含量,并通过蛋白免疫印迹法检测肝组织PDGF-BB蛋白及PDGFR蛋白的表达。结果与正常组相比,模型组羟脯氨酸含量、PDGF-BB蛋白及PDGFR蛋白的表达均明显增加(P<0.01);与模型组相比,中药组及秋水仙碱组羟脯氨酸含量减少(P<0.05),PDGF-BB蛋白及PDGFR蛋白的表达明显减少(P<0.01);中药组与秋水仙碱组羟脯氨酸含量、PDGF-BB蛋白及PDGFR蛋白的表达均未见明显差异(P>0.05)。结论大黄虫丸配伍黄芪、水红花子能有效拮抗猪血清诱导的大鼠免疫性肝纤维化,其机制可能与抑制PDGF-BB及受体PDGFR蛋白的表达,进而抑制肝星状细胞的增殖活化有关。Objective To investigate the influence of variation on Dahuang Zhechong pill combine with mikvetch root and prince＇ s-feather fruit decoction on PDGF protein and PDGFR protein of rats with hepatic fibrosis. Methods SD rats were randomly divided into normal group, model group and western medicine, Chinese medicine group. Except the normal group, the other 3 groups were induced liver fibrosis model by intraperitoneal injection of porcine serum, after six weeks of treatment, pathology HE of rat liver was conducted and alkali hydrolysis method was used to decect hydroxyproline content, the expression of PDGF - BB protein and PDGFR protein was detected by protein immunoblot method. Results Compared with the normal group, hydroxyproline content , PDGF-BB proteins and PDGFR protein expression of model group was significantly increased （ P〈0.01 ） ; Compared with model group, the hydroxyproline content in Chinese medicine group and colchicine group was decreased （P〈0.05） , the expression of PDGF-BB protein and PDGFR protein was decreased significantly （P〈0. 01 ）; the hydroxyproline content, the expression of PDGF-BB protein and PDGFR protein between Chinese medicine group and colchicine group had no significant difference （P〉0.05）. Conclusion variation on Dahuang Zhechong pill combine with mikvrtch root and prince＇ s-feather fruit decoction can antagonism liver fibrosis induced by porcine serum, the mechanism may be related to the inhibition of PDGF-BB and PDGFR protein expression, and the inhibition of the hepatic stellate cell activation.

关 键 词：大黄虫丸 免疫性肝纤维化 血小板源性生长因子 血小板源性生长因子受体 

分 类 号：R249[医药卫生—中医临床基础]