α-半乳糖苷酶转换人体肝脏血型的实验研究  

Blood type conversion in human liver - an experimental study on α-galactosidase

作　　者：田庆[1] 张建军[2] 邢雨[3] 李世朋[4] 王玉亮[5] 

机构地区：[1]寿光市人民医院普外三科,山东省262700 [2]天津市第一中心医院移植外科 [3]天津市第三中心医院普外科 [4]天津医科大学第一临床学院 [5]卫生部危重病急救医学重点实验室

出　　处：《中华肝胆外科杂志》2016年第10期677-681,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：天津市卫生局科技基金（2010KR03）

摘　　要：目的研究α-半乳糖苷酶对人体肝脏血型抗原的清除效果，探讨肝脏血型转换的可行性。方法采用肝移植手术中B血型病肝建立离体肝脏的灌注模型。用UW液＋／-α-半乳糖苷酶灌注离体肝脏，免疫荧光分析酶对肝脏B抗原的清除效果。结果用含α-半乳糖苷酶的uw液灌注离体肝脏，免疫组化显示，低温灌注保存后的肝脏血型抗原的含量明显降低。灌注后1ht3抗原减少至灌注前平均水平的58％、2h为10％、4h为4％。不同时间血型抗原水平差异有统计学意义（P〈0．05）。对照组血型抗原水平未见明显的变化。结论α-半乳糖苷酶可以有效清除人的离体肝组织B抗原。虽然并未达到不可测水平，但可基本实现人体器官肝脏B→O的血型转换，对研究人体器官的血型转换有重要意义。Objective To study blood type B antigen elimination with c^-galactosidase in human liv- er tissue, and discuss the feasibility of blood type conversion in human liver. Methods The liver specimens from patients with blood type B in liver transplantation were collected, and an in vitro liver peffusion model was established. The in vitro livers were perfused with UW solution ＋ / - α-galactosidase. The effect of en- zyme in B antigen of human liver were analyzed by immunofluorescence. Results With UW solution contai- ning α-galactosidase to perfuse the in vitro livers, immunohistochemistry showed the level of blood type B an- tigen in liver was significantly reduced after hypothermic perfusion and preservation. The B antigen level in 1 h perfusion was reduced to approximate 58% of this figure prior to perfusion, in 2 h was 10% , and in 4 h was 4%. Among the different intervals, the blood group antigen levels showed significant differences （P 〈 0. 05）. In the control group, the blood group antigen levels showed no obvious change on statistical analysis. Conclusions α-galactosidase was effective to clear blood type B antigen in isolated liver tissue. In the ex- perimental group, Although the B antigen did not fall to a undetectable level, liver blood type conversion from B→O remains a promising potential which has been meaningful for related researches on blood type conversion of human organs.

关 键 词：Α-半乳糖苷酶 人体 肝脏 血型转换 

分 类 号：R657.3[医药卫生—外科学]