冠状动脉狭窄时ST段压低动物模型的建立  

Establishment of an animal model of ST-segment depression in coronary artery stenosis

作　　者：辛卫朋[1] 陈士良[2] 陈国雄[1] 方波[1] 王红娜[1] 

机构地区：[1]浙江省舟山医院心内科,316000 [2]中国人民解放军第三〇五医院心内科

出　　处：《心电与循环》2016年第5期322-324,共3页Journal of Electrocardiology and Circulation

摘　　要：目的建立冠状动脉狭窄引起ST段压低的动物模型,探讨其病理生理改变。方法运用自制的微米缩窄器对16例犬的左旋支进行定量狭窄,观察左旋支血流量、冠状动脉远端小动脉平均压,左心室收缩峰压、左心室压力最大上升速率、左心室舒张末压、心肌耗氧量和乳酸的变化。结果当冠状动脉狭窄达87%(面积)时,可引起ST段压低(压低0.1m V),此时左旋支血流量降至(20±7)ml/min,冠状动脉远端小动脉平均压降至(55±8)mm Hg,与狭窄前[(36±6)ml/min、(90±14)mm Hg]比较,差异均有统计学意义(t=4.54、10.806,P<0.01),左心室收缩峰压和左心室压力最大上升速率[(106±18)mm Hg、(2 210±700)mm Hg/s]较狭窄前[(114±20)mm Hg、(2 450±760)mm Hg/s]降低,乳酸升高[(43.5±14)%比(26.4±6.9)%],心肌耗氧量降低[(2.22±0.64)ml/(100g·min)比(43.5±14.7)ml/(100g·min)],差异均有统计学意义(P<0.05或0.01)。结论在冠状动脉狭窄达87%时,可造成ST段压低的急性心肌缺血动物模型。Objective To establish an animal model of ST-segment depression induced by coronary artery stenosisand investigate its pathophysiological changes. Methods An animal model of ST-segment depression was establishedin 16 dogs by quantitatively constricting left circumflex coronary artery （LCCA） witha micrometer constrictor. The coronaryblood flow（CBF） and blood pressure of LCCA, left ventricular peak systolic pressure（LVPSP）, maximum rate of left ventricularpressure increase （dp/dt max）, left ventricular end diastolic pressure（LVEDP）, myocardial oxygen consumption and lactatewere measured. Results ST-segment depression developed as the degree of coronary stenosis reached to 87%（cross-sectional area） with CBF reduced from（36±6）ml/min to（20±7）ml/min, distal LCCA pressure decreased from（90±14）mmHg to（55±8）mmHg, LVPSP reduced from （114±20）mmHg to（106±18）mmHg and dp/dt max decreased from（2 450±760）mmHg/s to（2 210±700）mmHg/s）, lactate increased from（26.4±6.9）% to（43.5±14）%, myocardial oxygen consumptionreduced from（2.22±0.64）ml/（100g·min） to（1.37±0.83）ml/（100g·min）. The differences between the parameters beforeand after coronary narrowed were significant （all P〈0.05 or 0.01）. Conclusion It is needed for establishing an acutemyocardial ischemic animal model with ST segment depression to reduce 87%cross-sectional area of coronary artery.

关 键 词：冠状动脉狭窄 ST段压低 动物模型 

分 类 号：R541.4[医药卫生—心血管疾病]