结核患者外周血CD4^+CD25^+Foxp3^+Treg表达与巨噬细胞凋亡的临床意义  

The clinical significance of CD4^+CD25^+Foxp3^+ Treg expression and macrophage apoptosis in patients with tuberculosis

作　　者：周安[1] 徐巧玲[2] 李明强[1] 刘清蒙[1] 

机构地区：[1]遵义医学院微生物学教研室,贵州遵义563000 [2]贵州航天医院肺科,贵州遵义563000

出　　处：《重庆医学》2016年第30期4217-4219,共3页Chongqing medicine

基　　金：贵州省卫生厅基金资助项目(D-166)

摘　　要：目的探讨结核病患者外周血单个核细胞(PBMC)中CD4^+CD25^+Foxp3^+调节性T淋巴细胞(Treg)表达与单核巨噬细胞(MΦ)凋亡的可能关系及临床意义。方法用流式仪测定29例活动性结核、17例肺外结核患者治疗前及治疗后3、6个月PBMC中CD4^+CD25^+Foxp3^+Treg表达及MΦ凋亡率,并与20例健康对照者(健康对照组)进行比较。结果活动性结核患者PBMC中CD4^+CD25^+Foxp3^+Treg表达明显高于健康对照组和治疗后患者,且复治患者高于初治患者,肺外结核高于肺结核;患者MΦ凋亡率明显高于健康对照组(P<0.01),初治组明显高于复治组(P<0.01)。活动性结核患者PBMC中CD4^+CD25^+Foxp3^+Treg表达与MΦ凋亡呈负相关。结论 CD4^+CD25^+Foxp3^+Treg表达升高,MΦ凋亡下降,提示与结核病呈慢性感染有关。Objective To investigate the possible relationship between CD4^＋CD25^＋Foxp3^＋Treg expression and macrophage apoptosis in peripheral blood mononuclear cell（PBMC）in patients with tuberculosis and its clinical significance.Methods The expression of CD4^＋CD25^＋Foxp3^＋Treg before treatment and 3and 6months after treatment in PBMC and the apoptosis rate of monocytes in 29 patients with active tuberculosis and 17 patients with extrapulmonary tuberculosis were measured by flow cytometry,and the results were compared with those of 20 healthy controls（healthy control group）.Results The proportion of CD4^＋CD25^＋Foxp3^＋Treg in PBMC of active tuberculosis patients was significantly higher than that of controls and treated patients（P0.01）,and the proportion of re-treatment patients was higher than that of primary treatment patients.The proportion of CD4^＋CD25^＋Foxp3^＋Treg in PBMC from the extra-pulmonary tuberculosis patients was higher than that of pulmonary tuberculosis patients（P0.01）.The apoptosis rate of monocytes of patients with tuberculosis was higher than that of the control group（P0.01）,and the apoptosis rate of primary treatment patients were higher than that of re-treatment patients（P0.01）.The expression of CD4^＋CD25^＋Foxp3^＋Treg in patients with active tuberculosis was negatively correlated with the apoptosis of macrophages in PBMC.Conclusion The expression of CD4^＋CD25^＋Foxp3^＋Treg is increased,and the apoptosis of macrophages is decreased,which might be related to chronic infection of tuberculosis.

关 键 词：活动性结核 外周血单个核细胞 CD4^+CD25^+Foxp3^+调节性T淋巴细胞 单核巨噬细胞凋亡 

分 类 号：R52[医药卫生—内科学]