溺死大鼠肺组织AQP-1、AQP-4的表达  

Expression of AQP-1 and AQP-4 in the Lungs of Drown Rats

作　　者：赵兵[1] 姚士强[1] 郝小惠 

机构地区：[1]唐山市公安局法化大队,河北唐山063000 [2]华北理工大学医学实验研究中心,河北唐山063000

出　　处：《法医学杂志》2016年第5期321-325,共5页Journal of Forensic Medicine

基　　金：河北省科技厅科技支撑项目(10276116D)

摘　　要：目的观测生前溺死与死后抛入河水中大鼠肺组织水通道蛋白-1(aquaporin-1,AQP-1)、AQP-4的表达变化。方法将30只健康雄性Wistar大鼠随机分为河水溺死组、抛尸入水组及脱颈致死组。HE染色观察各组大鼠肺组织形态学变化,实时荧光定量PCR法检测各组大鼠肺组织AQP-1、AQP-4 m RNA的表达,免疫组织化学、Western印迹法检测其蛋白表达。结果免疫组织化学法和Western印迹法检测河水溺死组AQP-1蛋白的表达高于抛尸入水组和脱颈致死组(P<0.05)。免疫组织化学法显示河水溺死组AQP-4蛋白的表达高于抛尸入水组和脱颈致死组(P<0.05),而Western印迹法未检测出三者的差别(P>0.05)。河水溺死组大鼠肺组织的AQP-1、AQP-4 m RNA表达高于抛尸入水组(P<0.05)。结论河水溺死组的大鼠在急性肺损伤时肺组织AQP-1、AQP-4 mRNA及蛋白表达增高可能对生前溺死与死后抛尸的鉴别有一定意义。Objective To observe the changes of expression of aquaporin-1（AQP-1） and AQP-4 in drowned and postmortem immersed rats＇ lungs. Methods Thirty healthy male Wistar rats were randomly divided into drowning group, postmortem immersion group and cervical dislocation group. The morphological changes of rats＇ lungs were observed using HE staining. The m RNA and protein expressions of AQP-1 and AQP-4 in rats＇ lungs were detected by real-time PCR, immunohistochemistry and Western blotting, respectively. Results The results of immunohistochemistry and the Western blotting showed that the protein expression of AQP-1 of the drowning group was higher than the postmortem immersion group and the cervical dislocation group（P〈0.05）. The result of immunohistochemistry showed that the protein expression of AQP-4 of the drowning group was higher than the postmortem immersion group and the cervical dislocation group（P〈0.05） while no difference were detected among the three of them by Western blotting（P〉0.05）. The m RNA expressions of AQP-1 and AQP-4 in rats＇ lungs of the drowning group was significantly higher than the postmortem immersion group（P〈0.05）. Conclusion The increase of m RNA and protein expressions of AQP-1 and AQP-4 in lungs of rats with cute lung injury of the drowning group would be useful for differentiating vital drowning from postmortem immersion.

关 键 词：法医病理学 溺水 水通道蛋白质-1 水通道蛋白质-4 大鼠 

分 类 号：R135.2[医药卫生—劳动卫生]