基于紫外可见积分球反射光谱法鉴定血痕经历时间  

Determination of Bloodstain Age by UV Visible Integrating Sphere Reflection Spectrum

作　　者：闫立强 高野 

机构地区：[1]辽宁警察学院刑事技术系,辽宁大连116036

出　　处：《法医学杂志》2016年第5期326-328,共3页Journal of Forensic Medicine

基　　金：辽宁警察学院科研基金资助项目(2015Lnpckyjjxm002);公安部技术研究计划资助项目(2016JSYJ304)

摘　　要：目的建立一种快速鉴定血痕陈旧度的方法。方法在实验室条件(20℃、25℃、30℃)下,使用UV-2450型紫外可见分光光度计,利用反射附件ISR-240A积分球,以BaSO_4标准白板做参比,间隔一定时间测定健康人耳静脉血血痕纱布的反射光谱,采集特定波长反射率R_(541)、R_(577),计算R_(541)/R_(577)值,使用SPSS 17.0软件进行直线拟合与回归分析。结果一定条件下8 h内,血痕经历时间与其特定波长紫外可见反射率比值回归分析的R^2均大于0.8,回归方程成立,血痕经历时间与R_(541)/R_(577)值具有高度相关性。结论所建立的方法简便、快速、无损,可靠性好,可以对实验室条件下8 h内血痕经历时间进行鉴别。Objective To establish a method for rapid identification of bloodstain age. Methods Under laboratory conditions（20 ℃, 25 ℃ and 30 ℃）, an integrating sphere ISR-240A was used as a reflection accessory on an UV-2450 UV-vis spectrophotometer, and a standard white board of BaSO4 was used as reference, the reflection spectrums of bloodstain from human ears＇ venous blood were measured at regular intervals. The reflection radiosand R577at a specific wavelength were collected and the value of R541/R577was calculated. The linear fitting and regression analysis were done by SPSS 17.0. Results The results of regression analysis showed that R2 of the ratios of bloodstain age to UV visible reflectivity in specific wavelengths were larger than 0.8 within 8 hours and under certain circumstances. The regression equation was established. The bloodstain age had significant correlation with the value of R541/R577. Conclusion The method of inspection is simple, rapid and nondestructive with a good reliability, and can be used to identify the bloodstain age within 8 hours elapsed-time standards under laboratory conditions.

关 键 词：法医病理学 光谱分析 血痕 积分球 

分 类 号：R446.11[医药卫生—诊断学]