野生花生Hsp70基因家族的全基因组鉴定及生物信息学分析  

Genomic-wide identification and bioinformatics analysis of Hsp70 genes in Arachis duranensis and Arachis ipaёnsis

作　　者：陈湘瑜[1,2] 徐日荣[1,2] 熊发前[3] 唐兆秀[1,2] 

机构地区：[1]福建省农业科学院作物研究所,福建福州350013 [2]农业部闽台农作物种质资源利用重点开放实验室,福建福州350013 [3]广西农业科学院经济作物研究所,广西南宁530007

出　　处：《中国油料作物学报》2016年第5期572-581,共10页Chinese Journal of Oil Crop Sciences

基　　金：福建省科技计划项目(省属公益类科研院所基本科研专项;2015R1026-10)

摘　　要：热激蛋白70(heat shock protein 70,Hsp70)是细胞应对高温和其它胁迫环境时所产生的一类分子伴侣类型应激蛋白。本研究以Arachis duranensis和Arachis ipansis基因组数据库为基础,利用生物信息学方法对Hsp70基因家族进行鉴定分析。结果表明,A.duranensis含有34个Hsp70基因成员,A.ipansis含有35个Hsp70基因成员,两者的直系同源基因大部分分布于两套染色体相近的位置。Ad Hsp70和Ai Hsp70基因家族根据亚细胞定位结果可分4种类型,各类型的Hsp70基因结构相对保守。根据系统进化分析Hsp70基因家族可分为ClassⅠ(Hsp70)和ClassⅡ(Hsp110)两个亚家族,其中ClassⅠ亚家族进一步分为3个小家族,同时揭示Hsp70基因家族成员产生于单双子叶分化之前。GO分析预测表明,Ad Hsp70和Ai Hsp70基因家族功能类别完全一致,各功能类别数目比例变化相似。本研究结果有助于了解花生属植物Hsp70基因家族功能,以期为深入研究花生逆境生理过程中的分子调控机理提供基础。Heat shock protein 70 is a kind of molecular chaperones. They play an important role in cell responses to stresses. In this study,genome-wide identification and analysis of Hsp70 gene family members in Arachisduranensis and Arachis ipansis were performed using bioinformatic methods. The results showed that A. duranensis contained 34 Hsp70 genes,and A. ipansis contained 35 Hsp70 genes. The orthologous gene pairs located at the similar position in A. duranensis and A. ipansis. Ad Hsp70 and Ai Hsp70 family genes were classified into 3 types based on their sub-cellular locations,and the gene structures were relatively conserved in each type. Ad Hsp70 and Ai Hsp70 family genes were divided into ClassⅠ（Hsp70） subfamily and ClassⅡ（Hsp110） subfamily base on evolutionary analysis,and ClassⅠ subfamily was further divided into 3 small families. Evolutionary analysis of Hsp70 s in diverse species revealed that Hsp70 gene families occurred before the monocot and dicot differentiation. GO analysis showed a complete consistency between the function category of Ad Hsp70 gene family and Ai Hsp70 gene family,and the changes of function category proportion were similar. This study provided the complete profiles of genus Arachis Hsp70 gene family for future study on their functions related to the molecular mechanisms of stress physiology.

关 键 词：野生花生 Hsp70基因家族 生物信息学 系统发育 抗高温 

分 类 号：S565.203[农业科学—作物学]