Flower color mutants induced by carbon ion beam irradiation of geranium (Pelargonium × hortorum, Bailey)  

作　　者：Li-Xia Yu Wen-Jian Li Yan Du Gang Chen Shan-Wei Luo Rui-Yuan Liu Hui Feng Li-Bin Zhou 

机构地区：[1]Biophysics Group, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Road, Lanzhou 730000,China [2]University of Chinese Academy of Sciences, Beijing 100049,China [3]Ningxia Academy of Agriculture and Forestry Sciences, 590West Huanghe, Yinchuan 750002, China [4]Gansu Agricultural University, No. 1 Yingmen Village,Anning District, Lanzhou 730070, China

出　　处：《Nuclear Science and Techniques》2016年第5期152-159,共8页核技术（英文）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.11205218,11275171,and 11405234);the Knowledge Innovation Project of the Chinese Academy of Sciences(CAS)(No.KJCX2-EW-N05);CAS‘‘Light of West China’’Program(No.29Y506020);the Youth Innovation Promotion Association of CAS(No.29Y506030)supported this study

摘　　要：In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation,shoots of geranium were exposed at dosages of 0,10,15,30,and40 Gy,and one flower color mutant was obtained.The morphological characteristics,physiological aspects,and DNA polymorphisms between wild-type and flower color mutants were analyzed.The colors of petal,peduncle,pistil,and stamen of the mutant displayed significant differences compared to those of the wild-type.Compared to the original plants,the total anthocyanin content in the petals of the mutant significantly decreased,resulting in a light pink petal phenotype.DNA polymorphisms detected by random amplified polymorphic DNA analysis showed that the ratio of different bands between the wild-type and mutant reached up to 13.2%.The present study demonstrates that carbon ion beam irradiation is effective in inducing genomic variations,resulting in flower color geranium mutants within a relatively short period of time.Meanwhile,the developed flower-color mutants may be potentially used in future mutational research studies involving ornamental plants.In an attempt to elucidate the biological effects and underlying mutations involving flower color in ornamental plants following carbon ion beam radiation, shoots of geranium were exposed at dosages of 0, 10, 15, 30, and 40 Gy, and one flower color mutant was obtained. The morphological characteristics, physiological aspects, and DNA polymorphisms between wild-type and flower color mutants were analyzed. The colors of petal, peduncle, pistil, and stamen of the mutant displayed significant differences compared to those of the wild-type. Compared tothe original plants, the total anthocyanin content in the petals of the mutant significantly decreased, resulting in a light pink petal phenotype. DNA polymorphisms detected by random amplified polymorphic DNA analysis showed that the ratio of different bands between the wild-type and mutant reached up to 13.2 %. The present study demon-strates that carbon ion beam irradiation is effective in inducing genomic variations, resulting in flower color geranium mutants within a relatively short period of time. Meanwhile, the developed flower-color mutants may be potentially used in future mutational research studies involving ornamental plants.

关 键 词：总花色苷 碳离子束 突变体 天竺葵 离子束辐照 随机扩增多态性DNA分析 DNA多态性 香叶 

分 类 号：S682.19[农业科学—观赏园艺]