大鼠肾去交感神经实验动物模型的制备  

Establishment of a rat renal sympathetic denervation experimental animal model

作　　者：郑晓新[1] 汪小丁[1] 侯果[1] 邱璇[1] 蒋学俊[1] 

机构地区：[1]武汉大学人民医院心血管内科心血管病湖北省重点实验室武汉大学心血管病研究所,湖北省武汉市430060

出　　处：《中国心血管病研究》2016年第10期940-944,F0003,共6页Chinese Journal of Cardiovascular Research

基　　金：中央高校专项科研项目基金（项目编号：2042015kf0074）

摘　　要：目的 制备大鼠肾去交感神经实验动物模型.方法 52只SPF级SD大鼠随机分为正常对照组（N组）、肾去交感组（RDN组）、心肌梗死组（MI＋Sham组）和心肌梗死加肾去交感治疗组（MI＋RDN组）四组.MI＋Sham组和MI＋RSD组分别结扎前降支冠脉制备心梗模型,7d后分别予肾去交感假手术或手术处理.电刺激法评估术前及RDN术后即刻,HE染色法评估术前和MI术后4周肾交感去除情况,ELISA法评估血浆血管紧张素Ⅱ（AngⅡ）、醛固酮（ALD）和去甲肾上腺素（NE）变化情况.结果 RDN术总死亡率为18.1％,手术成功率为81.9％.在RDN术前,肾神经干电刺激可诱导明显交感反应,而术后上述反应消失.HE染色显示,正常未消融的肾动脉外膜外可见完整交感神经,RDN术后3周未见任何完整肾交感神经,肾动脉血管壁未见明显异常.与MI＋Sham组相比,MI＋RDN组AngⅡ、ALD及NE均明显减少[NE：（669.0±24.0）ng/L比（1349.0±209.0）ng/L,P＜0.01;AngⅡ：（205.5±16.4）ng/L比（1069.0±209.1）ng/L,P＜0.01;ALD：（290.0±36.0）ng/L比（1049.5±298.5）ng/L,P＜0.01].结论 本研究所制备的大鼠肾去交感模型具有良好的操作性、安全性、实用性和有效性.Objective To establish a repeatable and steady rat renal sympathetic denervation （RDN） model. Methods 52 SPF level SD rats were randomly divided into 4 groups, N group, RDN group, MI＋Sham group and MI＋RSD group. Bilateral renal sympathectomy or sham operation were performed in RDN group and MI＋ RSD group or MI＋Sham group, separately. Electrical stimulation and HE staining method were used to assess the situation of renal sympathetic denervation at the preoperative and/or postoperative and 4 weeks post-MI, ELISA method was adopted to evaluate the plasma levels of Angiotensin Ⅱ （Ang Ⅱ ）, Aldosterone （ALD） and Norepinephrine （NE）. Results 22 rats were used for renal sympathetic operation, the total RDN mortality was 18.1% and the RDN success rate was 81.9%. Electrical stimulation induced to obvious sympathetic response pre-operation of RDN, and the above reaction disappeared after RDN. HE staining showed the complete sympathetic nerve outside the outer membrane of the renal arteries, which could not be observed 4 weeks post-RDN, renal artery walls were normal at that time. Compared with MI＋Sham group, the plasma levels of Ang Ⅱ , ALD and NE were signif- icantly decreased in MI＋RDN group （P〈0.01）. Conclusion Rat renal sympathetic animal model made in this study, has a good operability, security, practicability and effectiveness. It can be used to basic studies to evaluate renal sympathetic related diseases.

关 键 词：大鼠 肾去交感 化学消融 模型 

分 类 号：Q95-33[生物学—动物学] R542.22[医药卫生—心血管疾病]