固相萃取-高效液相色谱-荧光法测定普萘洛尔微乳凝胶给药后家兔血药浓度  

Determination of microemulsion gel of propranolol in rabbit plasm by solid phase extraction and HPLC with fluorescence detector

作　　者：温双双[1] 汪杰[2] 李雅潇[3,4] 崔颖[3,4] WEN Shuang-shuang WANG Jie LI Ya-xiao CUI Ying(Department of Pharmaceutical, Yantaishan Hospital, Yantai 26400, Chin)

机构地区：[1]山东省烟台市烟台山医院药剂科,山东烟台264000 [2]武警部队药品仪器检验所,北京102600 [3]武警后勤学院药物化学教研室,天津300309 [4]天津市职业危害与防制重点实验室,天津300309

出　　处：《武警后勤学院学报（医学版）》2016年第8期610-613,共4页Journal of Logistics University of PAP（Medical Sciences）

摘　　要：【目的】建立普萘洛尔微乳凝胶给药后家兔体内血药浓度的侧定方法。【方法】采用高效液相色谱-荧光法测定普茶洛尔血药浓度,用Supelco C18小柱萃取血浆中的普茶洛尔,色谱柱为phenomenex ODS（250 mm×4.6 mm,5μm）,流动相为甲醇-0.05 mol/L的碳酸氨水溶液（40：60）,流速1.0 ml/min。【结果】普茶洛尔血药浓度在1.0~50.0 ng/ml内线性良好,r2=0.992;不同浓度方法回收率分别为89.93%,93.13%和91.30%。日内和日间精密度RSD分别小于2.56%和5.02%。【结论】建立了简单、快速和准确的家兔体内普茶洛尔经皮血药浓度的固相萃取和高效液相色谱-荧光测定方法。【Objective】To establish a method for determining the microemulsion gel of propranolol in rabbit plasm.【Method】The plasm propranolol was pretreated by a Supelco C18 cartridge and separated on a phenomenex ODS column（250 mm×4.6 mm, 5μm）.The mobile phase consisted of methanol 0.05 mol/L ammonium hydrogen carbonate solution（40：60） at a flow-rate of 1.0 ml/min. The plasm concentration of propranolol was determined by HPLC with fluorescence detector.【Result】The plasm concentration of propranolol showed good linearity in the range of 1.0~50.0 ng/ml and the correlation coefficient was 0.992. The recovery rates in different concentrations were respectively 89.93%, 93.13% and 91.30%. The intra-day and inter-day RSDs were less than 2.56% and5.02%.【Conclusion】A simple, rapid and precise method is established for determining the concentration of transdermal propranolol in rabbit plasm by solid phase extraction and HPLC with flurescence detector.

关 键 词：普萘洛尔 固相萃取 经皮 荧光 

分 类 号：R96[医药卫生—药理学]