附子、白及反药组合对乙酸诱导大鼠胃溃疡的影响及其机制研究  

Effect of compatibility of Aconiti Lateralis Radix Preparata and Bletillae Rizoma anti drug combination on acetic acid induced gastric ulcer in rats and its mechanism

作　　者：孙萌[1] 卢志强[1,2] 庄朋伟[1] 张艳军[1] 

机构地区：[1]天津中医药大学中药学院天津市中药药理重点实验室,天津300193 [2]天津大学药物科学与技术学院,天津300072

出　　处：《现代药物与临床》2016年第9期1319-1324,共6页Drugs & Clinic

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB505300;2011CB505302)

摘　　要：目的观察附子、白及反药组合对乙酸诱导胃溃疡大鼠的毒效表现,并基于β2肾上腺素能受体(β2-AR)探讨其作用机制。方法大鼠随机分为对照组、模型组、沙丁胺醇组、普萘洛尔组、白及组、附子组和反药组合组。乙酸贴敷法制备胃溃疡模型,ig给药,观察溃疡面积、胃黏膜病理形态变化。利用Elisa试剂盒检测胃组织匀浆炎症相关指标髓过氧化物酶(MPO)、白细胞介素6(IL-6)、环磷酸腺苷(c AMP)水平变化。采用双荧光素酶报告基因技术评价附子、反药组合对β2-AR的激动作用。结果与模型组比较,附子组、反药组合组溃疡严重、自愈不佳,差异有统计学意义(P<0.05);白及组溃疡较轻,愈合明显,差异有极显著意义(P<0.01)。与模型组比较,反药组合组IL-6、MPO、c AMP水平均显著升高(P<0.05、0.01),附子组MPO水平显著升高(P<0.01)。附子具有明显的激动β2-AR作用,与白及联合使用后激动作用进一步增强(P<0.01)。结论附子、白及反药组合加重胃溃疡和炎症反应是其"相反"的主要表现,其机制可能与协同激动β2肾上腺素能受体有关。Objective To observe toxic effect performance of Aconiti Lateralis Radix Preparata and Bletillae Rizoma anti drug combination under the conditions of acetic acid induced gastric ulcer in rats, and explore its mechanism based onβ2 adrenergic receptor （β2-AR）. Methods Rats were randomly divided into normal group, model group, salbutamol group, propranolol group, Aconiti Lateralis Radix Preparata group, Bletillae Rizoma group, and anti drug combination group. Acetic acid was applied to make gastric ulcer model, and area of gastric ulcer and pathological morphological changes were observed after filling the stomach with medicines. Indicators related with homogenate inflammation MPO, IL-6, and cAMP changes in stomach tissue were determined by Elisa kits method. Activating actions onβ2-AR of Aconiti Lateralis Radix Preparata and Bletillae Rizoma anti drug combination were evaluated with technology of double luciferase report gene. Results Compared with model group, ulcer of Aconiti Lateralis Radix Preparata and anti drug combination group were serious, the self healings were not good （P〈0.05）, but ulcers of Bletillae Rizoma group were light, and the healings were obvious （P 〈 0.01）. Compared with model group, levels of IL-6, MPO, and cAMP in anti drug combination group were significantly increased （P〈0.05, 0.01）, and levels of MPO in Aconiti Lateralis Radix Preparata group were also significantly increased （P〈0.01）. Aconiti Lateralis Radix Preparata had obvious activating action onβ2-AR, and this action had further enhanced after combination with Bletillae Rizoma （P〈0.01）. Conclusion Aconiti Lateralis Radix Preparata and Bletillae Rizoma anti drug combination has aggravating stomach ulcers of acetic acid burning type, and inflammation is the main“contrary”reflection, whose mechanism may be related to collaborative excitedβ2 receptor.

关 键 词：附子 白及 胃溃疡 溃疡面积 Β2肾上腺素能受体 

分 类 号：R285.5[医药卫生—中药学]