不同糖种类及糖水平对松浦镜鲤肠道消化酶活性及肠道和肝脏组织结构的影响  

Effects of Different Carbohydrate Types and Carbohydrate Levels on Intestinal Digestive Enzyme Activities,Intestinal and Liver Histological Structure of Songpu Mirror Carp(Cyprinus carpio specularis)

作　　者：李晋南[1] 王常安[1] 王连生[1] 赵志刚[1] 罗亮[1] 都雪[1] 徐奇友[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,哈尔滨150070

出　　处：《动物营养学报》2016年第10期3217-3224,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：中央级公益性科研院所基本科研业务费专项(HSY201306);现代农业产业技术体系建设专项(CARS-46)

摘　　要：本研究旨在探讨不同糖种类及糖水平对松浦镜鲤肠道消化酶活性及肠道和肝脏组织结构的影响。以酪蛋白和鱼粉为主要蛋白质源,鱼油和豆油为脂肪源,葡萄糖和淀粉分别为糖源,配制糖水平分别为25.0%和50.0%的4种等氮等脂的试验饲料。选用初始体重为(8.30±0.15)g的松浦镜鲤420尾,随机分为4组,每组3个重复,每个重复35尾鱼。4组试验鱼饲喂2种糖类型和2种糖水平的4种等氮等脂的试验饲料,分别命名为低淀粉(LS)组、高淀粉(HS)组、低葡萄糖(LG)组和高葡萄糖(HG)组。试验期为60 d。结果表明:HS组的肠道淀粉酶活性显著高于其他各组(P<0.05),蛋白酶活性显著高于HG组和LG组(P<0.05);HG组和LG组的肠道脂肪酶活性显著高于LS组(P<0.05)。HG组的前肠皱襞高度显著低于LG组(P<0.05),中肠皱襞高度显著低于LS组(P<0.05),后肠皱襞高度显著低于其他各组(P<0.05)。各组前肠、中肠和后肠肌层厚度没有显著差异(P>0.05)。HG组的前肠和中肠皱襞宽而短,皱襞密度低于LS组和LG组。HS组和HG组的肝细胞膜破裂,出现空泡现象。综上,含50%淀粉的饲料可增加松浦镜鲤肠道消化酶活性,含50%葡萄糖的饲料则会降低肠道皱襞高度,且饲料糖水平为50%时对松浦镜鲤肝脏组织结构造成了一定程度的负面影响。This experiment was aimed to study the effects of different carbohydrate types and carbohydrate levels on intestinal digestive enzyme activities,intestinal and liver histological structure of Songpu mirror carp（Cyprinus carpio specularis）.A total of 420 Songpu mirror carp with the average body weight of（8.30 ± 0.15） g were randomly divided into 4 groups with 3 replicates and 35 fish per replicate.The fish in 4 groups were fed 4 isonitrogenous and isolipidic experimental diets with two carbohydrate types（starch and glucose） and two carbohydrate levels（25.0 % and 50.0 %）,and the groups were named as low starch（LS） group,high starch（HS） group,low glucose（LG） group and high glucose（HG） group,respectively.The experiment lasted for 60 days.The results showed that the intestinal amylase activity of HS group was significantly higher than that of other groups（P〈0.05）.The intestinal protease activity of HS group was significantly higher than that of HG and LG groups（P〈0.05）.The intestinal lipase activity of HG and LG groups was significantly higher than that of LS group（P〈0.05）.The fold height of foregut of HG group was significantly lower than that of LG group（P〈0.05）.The fold height of midgut of HG group was significantly lower than that of LS group（P〈0.05）.The fold height of hindgut of HG group was significantly lower than that of other groups（P〈0.05）.The muscular thickness of foregut,midgut and hindgut had no significant difference among groups（P〉0.05）.The folds of foregut and midgut of HG group was wide and short,and the fold density of foregut and midgut was lower than that of LS and LG groups.The membranes of hepatocyte of HS and HG groups were ruptured and demonstrated vacuolation.In conclusion,dietary contained 50 % starch can improve intestinal digestive enzyme activities in Songpu mirror carp,but dietary contained 50 % glucose can decrease the intestine fold height,and 50 % dietary carbohydrate level has a negative impact on the histologi

关 键 词：松浦镜鲤 葡萄糖 淀粉 肠道形态 肝脏 

分 类 号：S963[农业科学—水产养殖]