敌草快和硫辛酸对育肥猪肠道结构及消化功能的影响  

Effects of Diquat and Lipoic Acid on Intestinal Morphology and Digestive Function of Finishing Pigs

作　　者：鲍伟光[1] 郝月[1] 崔艳军[1] 李洁蕾 张校军[1] 李淦[1] 王占彬[2] 顾宪红[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京100193 [2]河南科技大学动物科技学院,洛阳471003

出　　处：《动物营养学报》2016年第10期3264-3274,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点基础研究发展计划课题(2012CB124706);国家科技支撑计划课题(2012BAD39B02);中国农业科学院科技创新工程(ASTIP-IAS07)

摘　　要：本文旨在研究敌草快和硫辛酸(LA)对育肥猪肠道结构及消化功能的影响,以探究LA对敌草快引起的应激是否有缓解作用。选取24头(70.64±3.61)kg的健康大白阉公猪,按照2×2因子设计,随机分为对照组、LA组、敌草快组和LA+敌草快组,每个组设6个重复,每个重复1头猪。试验期29 d。LA添加剂量为800 mg/kg饲粮,饲喂贯穿试验全程,而一次性腹腔滴注敌草快于试验第15天进行,无腹腔滴注敌草快试验猪滴注等量的生理盐水。于试验第29天对所有试验猪前腔静脉采血后屠宰取样,运用试剂盒检测血浆和肠道氧化损伤标志物的含量以及空肠食糜消化酶的活性,苏木精-伊红(HE)染色后运用图像分析软件观察肠道形态结构并测定绒毛高度、隐窝深度,并计算绒毛高度/隐窝深度(V/C),荧光定量PCR法检测闭合蛋白(occludin)和紧密结合蛋白(ZO-1)的相对表达量。结果表明:1)敌草快极显著升高了试验猪血浆和肠道丙二醛(MDA)、蛋白质羰基(PCO)和8-羟基鸟苷(8-OHd G)的含量(P<0.01),极显著降低了十二指肠、空肠及回肠绒毛高度、隐窝深度(P<0.01),极显著增大了十二指肠和空肠V/C(P<0.01),显著增大了回肠V/C(P<0.05),极显著降低了空肠occludin和ZO-1 m RNA相对表达量(P<0.01),极显著降低了空肠食糜中淀粉酶、胰蛋白酶以及脂肪酶的活性(P<0.01)。2)饲粮中添加LA对试验猪血浆和肠道MDA、PCO和8-OHd G含量的影响均不显著(P>0.05),对十二指肠、空肠和回肠的绒毛高度、隐窝深度以及V/C的影响均不显著(P>0.05),对空肠occludin和ZO-1 m RNA相对表达量的影响也不显著(P>0.05),对空肠食糜中淀粉酶、胰蛋白酶以及脂肪酶活性的影响差异也均不显著(P>0.05)。3)在应激状态下,饲粮中添加LA使猪血浆8-OHd G含量显著降低(P<0.05),十二指肠绒毛高度、空肠绒毛高度以及回肠隐窝深度显著升高(P<0.05),空肠V/C显著降低(P<0.05),回肠V/C极显著降低(P<0.01),空This study was to investigate the effects of diquat and lipoic acid（LA） on intestinal morphology and digestive function of finishing pigs,in order to explore whether diquat induced stress on the LA had a role in alleviating.Twenty-four healthy finishing pigs（Yorkshire） with the body weight of（70.64 ± 3.61） kg were randomly allocated to control,LA,diquat,LA ＋ diquat groups with 6 replicates per group and 1 pig per replicate by a 2 × 2 factorial arrangement.The trial period was 29 days.LA was given all in the trial（800 mg/kg diet）.Diquat challenged pigs were given a single intraperitoneal injection of diquat at the 15 th days and non-challenged pigs were injected intraperitoneally with the equal physiological saline amount at the same time.All the pigs were slaughtered after the vena cava blood was sampledon the 29 th day.Using kits to detected the contents of plasma and intestine oxidative damage markers and the activities of digestive enzymes in jejunum chyme.After hematoxylin and eosin（HE） staining,using image analysis software to observed intestinal morphology structure and determined villus height,crypt depth,and calculated the value of the villus height to crypt depth（V/C）.Using fluorescence quantitative PCR to detected the relative expression abundance of occludin and zonula occludens protein-1（ZO-1） m RNA in jejunum.The results showed as follows： 1） diquat significantly increased the contents of malondialdehyde（MDA）,protein carbonyl（PCO） and 8-hydroxy deoxyguanosine（8-OHd G） in plasma and intestine（P〈0.01）,significantly reduced the villus height and crypt depth in duodenum and jejunum and ileum（P〈0.01）,while significantly increased the V/C in duodenum and jejunum（P〈0.01）,and significantly increased the V/C of the ileum（P〈0.05）,significantly reduced the relative expression abundance of occludin and ZO-1 m RNA in jejunum（P〈0.01）,and significantly reduced the activities of amylase,trypsase and lipase in jejunum chyme（P〈0.01）.2�

关 键 词：敌草快 硫辛酸 育肥猪 氧化应激 肠道 

分 类 号：S828[农业科学—畜牧学]