Optimization of dosage ratio of chlorogenic acid and gardenia glycosides in the treatment of rats with fatty liver disease induced by high-fat feed  

作　　者：Liang Huiqing Yang Jiaen Tang Jinmo Wu Chuncheng Li Hongshan Chen Shaodong 

机构地区：[1]Department of Liver Disease, Xiamen Hospital of Traditional Chinese Medicine [2]Department of Liver Disease, Ningbo No. 2 Hospital [3]Department of Traditional Chinese Medicine, Medical College of Xiamen University

出　　处：《Journal of Traditional Chinese Medicine》2016年第5期683-688,共6页中医杂志（英文版）

基　　金：Supported by the General Project of National Natural Science Foundation of China,Research of the Ratio Optimization between Chlorogenic Acid and Geniposide for Non-alcoholic Fatty Liver Disease,the Mechanism of Action for Epithelial-Mesenchymal Transition(No.81274155),Mechanism of Chlorogenic Acid and Geniposide for NASH by Regulating Kupffer Cells Polarization Based on Gut-liver Axis(No.81673660),the Youth Project of National Natural Science Foundation of China,Research on the mechanism of compound prescription of Chinese traditional medicine regulating endocannabinoid system in non-alcoholic steatohepatitis(No.81503529),Mechanism of treatment of nonalcoholic fatty liver disease by"HJJB"compound of Chinese traditional medicine based on insulin transduction(No.81503404);the Pilot Project of Science and Technology of Fujian Province,Study of Compound Prescription of Chinese Traditional Medicine on Fibrosis Based on Endocannabinoid System(No.2016D012);the TCM Project of Fujian Health Department,Clinic Research of Chinese Traditional Medicine for Nonalcoholic Fatty Liver(No.wzpw201308);the General Project of Xiamen Science and Technology Program Grant,Research of Chinese Traditional Medicine with IFNαTreatment on CHB(No.3502Z20134020)

摘　　要：OBJECTIVE: To investigate the optimal dosage ratio of chlorogenic acid and gardenia glycosides in treating the rates with fatty liver disease induced by high-fat feed.METHODS: A rat model of non-alcoholic fatty liver disease(NAFLD) was established by using a high-fat diet. According to mathematical model "uniform design", varying doses of chlorogenic acid and gardenia glycosides have been combined to form 6 medications for the treatment of NAFLD.Samples were then taken to observe pathological changes of the liver tissue(HE staining); changes in the fat metabolism pathway e.g. triglyceride(TG)and free fatty acid(FFA) content; alterations in liver function, i.e. serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST) activity; and differences in Malondialdehyde(MDA) and superoxide dismutase(SOD) content in the liver tissue. Multiple regression analysis was conducted to test the optimal dosage ratio of chlorogenic acid and gardenia glycosides.RESULTS: Fatty degeneration and vacuole-like changes of different degrees occurred in hepatic cells of the model group. Markers for fat metabolism, serum ALT and AST activities, and expression of MDA in liver tissue significantly increased, while SOD decreased. Combination of 90 mg chlorogenic acid and 90 mg Gardenia glycosides was the optimal dosage ratio of chlorogenic acid and gardenia glycosides in the treatment of rats with fatty liver induced by high-fat diet.CONCLUSION: Chlorogenic acid of 90 mg plus gardenia glycosides of 90 mg was the best combination in the treatment of fatty liver disease in rats induced by high-fat feed.OBJECTIVE： To investigate the optimal dosage ratio of chlorogenic acid and gardenia glycosides in treating the rates with fatty liver disease induced by high-fat feed.METHODS： A rat model of non-alcoholic fatty liver disease（NAFLD） was established by using a high-fat diet. According to mathematical model ＂uniform design＂, varying doses of chlorogenic acid and gardenia glycosides have been combined to form 6 medications for the treatment of NAFLD.Samples were then taken to observe pathological changes of the liver tissue（HE staining）; changes in the fat metabolism pathway e.g. triglyceride（TG）and free fatty acid（FFA） content; alterations in liver function, i.e. serum alanine aminotransferase（ALT）and aspartate aminotransferase（AST） activity; and differences in Malondialdehyde（MDA） and superoxide dismutase（SOD） content in the liver tissue. Multiple regression analysis was conducted to test the optimal dosage ratio of chlorogenic acid and gardenia glycosides.RESULTS： Fatty degeneration and vacuole-like changes of different degrees occurred in hepatic cells of the model group. Markers for fat metabolism, serum ALT and AST activities, and expression of MDA in liver tissue significantly increased, while SOD decreased. Combination of 90 mg chlorogenic acid and 90 mg Gardenia glycosides was the optimal dosage ratio of chlorogenic acid and gardenia glycosides in the treatment of rats with fatty liver induced by high-fat diet.CONCLUSION： Chlorogenic acid of 90 mg plus gardenia glycosides of 90 mg was the best combination in the treatment of fatty liver disease in rats induced by high-fat feed.

关 键 词：Chlorogenic acid Gardenia glycosides Non-alcoholic fatty liver disease Uniform design method Dose optimization 

分 类 号：R285.5[医药卫生—中药学]